犬视网膜视杆细胞G蛋白转导α、β1和γ1亚基编码cDNA的克隆与分析

Cloning and analysis of the cDNA encoding the rod G-protein transduction alpha, beta1 and gamma1 subunits from the canine retina.

作者信息

Kylmä T, Paulin L, Hurwitz M Y, Hurwitz R L, Kommonen B

机构信息

Department of Clinical Sciences/Surgery, Faculty of Veterinary Medicine, University of Helsinki, Finland.

出版信息

Gene. 1997 Jul 1;193(1):1-4. doi: 10.1016/s0378-1119(97)00060-7.

DOI:10.1016/s0378-1119(97)00060-7

PMID:9249060

Abstract

The canine (Canis familiaris) retinal rod transducin (G(T)) alpha, beta1 and gamma1 subunits were sequenced. Cloning of the cDNAs was accomplished by polymerase chain reaction (PCR) using degenerate and wild type retinal cDNA libraries as templates. The deduced amino acid sequences were highly similar to rod transducins from other species: G(T alpha) differed by 5 amino acids from the corresponding human sequence, whereas beta1 and gamma1 were identical to human sequences. The coding sequence of rod transducin was evaluated as a possible cause for the recessively inherited retinal rod-cone degeneration: there were no nucleotide differences between the wild type and retinal degenerate strains.

摘要

对犬（犬属家犬）视网膜视杆转导蛋白（G(T)）的α、β1和γ1亚基进行了测序。以简并和野生型视网膜cDNA文库为模板，通过聚合酶链反应（PCR）完成了cDNA的克隆。推导的氨基酸序列与其他物种的视杆转导蛋白高度相似：G(Tα)与相应的人类序列有5个氨基酸不同，而β1和γ1与人类序列相同。对视杆转导蛋白的编码序列进行评估，以确定其是否为隐性遗传视网膜视杆-视锥细胞变性的可能原因：野生型和视网膜变性菌株之间没有核苷酸差异。