从视网膜退化的拉布拉多寻回犬中克隆编码视杆光感受器环鸟苷酸磷酸二酯酶α和γ亚基的cDNA

Cloning of the cDNA encoding rod photoreceptor cGMP-phosphodiesterase alpha and gamma subunits from the retinal degenerate Labrador retriever dog.

作者信息

Kylmä T, Paulin L, Hurwitz M Y, Hurwitz R L, Kommonen B

机构信息

Department of Clinical Sciences/Surgery, Faculty of Veterinary Medicine, University of Helsinki, Finland.

出版信息

Res Vet Sci. 1997 May-Jun;62(3):293-6. doi: 10.1016/s0034-5288(97)90208-3.

DOI:10.1016/s0034-5288(97)90208-3

PMID:9300552

Abstract

The nucleotide (nt) sequence of the cDNA encoding the retinal rod cyclic 3'5'-GMP phosphodiesterase (PDE) alpha and gamma subunits from two strains of dogs-(i) Labrador Retrievers homozygous for autosomally recessively inherited rod-cone degeneration and (ii) the wild-type Beagle-are reported. Cloning of these subunits was accomplished by polymerase chain reaction using retinal cDNA libraries as templates. The nt sequence of alpha PDE predicts a 861-amino-acid polypeptide which is 97.7 per cent and 96.9 per cent identical to the bovine and human counterparts, respectively. PDE gamma encodes an 87-amino-acid polypeptide differing from bovine and murine gamma subunits by only one amino acid. Since no differences were found between these two strains of dogs, the cause of the Labrador Retriever's degeneration remains to be determined.

摘要

报道了来自两种犬类的编码视网膜视杆细胞环磷酸鸟苷3',5'-磷酸二酯酶（PDE）α和γ亚基的cDNA的核苷酸（nt）序列——（i）因常染色体隐性遗传的视杆-视锥细胞变性而纯合的拉布拉多寻回犬，以及（ii）野生型比格犬。这些亚基的克隆是通过聚合酶链反应，以视网膜cDNA文库为模板完成的。α-PDE的nt序列预测出一种861个氨基酸的多肽，分别与牛和人的对应多肽有97.7%和96.9%的同一性。PDEγ编码一种87个氨基酸的多肽，与牛和鼠的γ亚基仅相差一个氨基酸。由于在这两种犬类之间未发现差异，拉布拉多寻回犬变性的原因仍有待确定。