Lillard J W, Fetherston J D, Pedersen L, Pendrak M L, Perry R D
Department of Microbiology and Immunology, University of Kentucky, Lexington 40536-0084, USA.
Gene. 1997 Jul 1;193(1):13-21. doi: 10.1016/s0378-1119(97)00071-1.
We have sequenced a region from the pigmentation (pgm) locus of Yersinia pestis KIM6+ that is identified with the exogenous hemin storage (Hms+) phenotype in cells grown at 26 but not at 37 degrees C. The hmsHFRS locus encodes a putative polycistronic operon, with hmsH encoding an outer membrane protein with a deduced molecular mass of 93.4/89.5 (unprocessed/processed) kDa. The mature HmsH 788 aa polypeptide has a pI of 4.99. The hmsF gene has an open reading frame of 654 aa, encoding a 74.6/72.2 kDa OM protein with a pI of 5.16 when processed. A deduced 423 aa, 52 kDa protein with a pI of 10.83 is encoded by hmsR. HmsR has a basic, hydrophilic, and alpha-helical carboxyl terminus; 13 aa at the amino-terminal end and a 'KRKRAR' sequence at the carboxy-terminal end are essential for an Hms+ phenotype. The hmsS gene encodes a hypothetical 155 aa, 17.5 kDa protein with a pI of 6.68. Hms- Y. pestis strain M23-2 transformed with the cloned hmsHFRS locus developed an Hms(c) phenotype (Hms+ at 26-37 degrees C) due to mutations in genes outside the pgm locus.
我们对鼠疫耶尔森氏菌KIM6 +的色素沉着（pgm）位点的一个区域进行了测序，该区域在26℃而非37℃培养的细胞中与外源性血红素储存（Hms +）表型相关。hmsHFRS位点编码一个假定的多顺反子操纵子，其中hmsH编码一种外膜蛋白，推导的分子量为93.4 / 89.5（未加工/加工）kDa。成熟的HmsH 788个氨基酸的多肽的pI为4.99。hmsF基因有一个654个氨基酸的开放阅读框，编码一种74.6 / 72.2 kDa的外膜蛋白，加工后的pI为5.16。hmsR编码一个推导的423个氨基酸、52 kDa的蛋白，pI为10.83。HmsR具有一个碱性、亲水性的α-螺旋羧基末端；氨基末端的13个氨基酸和羧基末端的“KRKRAR”序列对于Hms +表型至关重要。hmsS基因编码一种假定的155个氨基酸、17.5 kDa的蛋白，pI为6.68。用克隆的hmsHFRS位点转化的Hms - 鼠疫耶尔森氏菌菌株M23 - 2由于pgm位点以外的基因突变而产生了Hms（c）表型（在26 - 37℃为Hms +）。
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