用于检测豚鼠腺病毒的聚合酶链反应

Polymerase chain reaction for detection of guinea pig adenovirus.

作者信息

Pring-Akerblom P, Blazek K, Schramlová J, Kunstýr I

机构信息

Institute of Virology and the Central Laboratory Animal Facility, Medical School Hannover, Germany.

出版信息

J Vet Diagn Invest. 1997 Jul;9(3):232-6. doi: 10.1177/104063879700900302.

DOI:10.1177/104063879700900302

PMID:9249160

Abstract

Lack of in vitro cultivation methods has inhibited the development of rapid, reliable diagnostic procedures for adenovirus-associated necrotizing bronchopneumonia in guinea pigs. Because polymerase chain reaction (PCR) techniques are well established for human adenoviruses, primers for the amplification of guinea pig adenovirus DNA were evaluated. The DNA for PCR was purified from the lung tissue of spontaneously infected and healthy guinea pigs. Adenovirus DNA could only be detected in the lungs of the infected animals. Subsequent sequence analysis of PCR products revealed that the guinea pig adenovirus is a distinct adenovirus.

摘要

缺乏体外培养方法阻碍了针对豚鼠腺病毒相关性坏死性支气管肺炎快速、可靠诊断程序的开发。由于聚合酶链反应（PCR）技术已广泛应用于人类腺病毒，因此对用于扩增豚鼠腺病毒DNA的引物进行了评估。用于PCR的DNA是从自然感染和健康豚鼠的肺组织中纯化得到的。仅在受感染动物的肺中检测到腺病毒DNA。随后对PCR产物的序列分析表明，豚鼠腺病毒是一种独特的腺病毒。