Thomson Darelle, Meers Joanne, Harrach Balázs
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
Virus Res. 2002 Feb 26;83(1-2):189-95. doi: 10.1016/s0168-1702(01)00437-3.
Partial genome characterisation of a non-cultivable marsupial adenovirus is described. Adenovirus-like particles were found by electron microscopy (EM) in the intestinal contents of brushtail possums (Trichosurus vulpecula) in New Zealand. Using degenerate PCR primers complementary to the most conserved genome regions of adenoviruses, the complete nucleotide sequence of the penton base gene, and partial nucleotide sequences of the DNA polymerase, hexon, and pVII genes were obtained. Phylogenetic analysis of the penton base gene strongly suggested that the brushtail possum adenovirus (candidate PoAdV-1) belongs to the recently proposed genus Atadenovirus. Sequence analysis of the PCR products amplified from the intestinal contents of brushtail possums originating from different geographical regions of New Zealand identified a single genotype. This is the first report of molecular confirmation of an adenovirus in a marsupial.
本文描述了一种不可培养的有袋类腺病毒的部分基因组特征。通过电子显微镜（EM）在新西兰帚尾袋貂（Trichosurus vulpecula）的肠道内容物中发现了腺病毒样颗粒。使用与腺病毒最保守基因组区域互补的简并PCR引物，获得了五邻体基部基因的完整核苷酸序列，以及DNA聚合酶、六邻体和pVII基因的部分核苷酸序列。对五邻体基部基因的系统发育分析强烈表明，帚尾袋貂腺病毒（候选PoAdV-1）属于最近提出的Atadenovirus属。对从新西兰不同地理区域的帚尾袋貂肠道内容物中扩增的PCR产物进行序列分析，确定为单一基因型。这是有袋类动物中腺病毒分子确认的首次报道。
