New Hampshire Veterinary Diagnostic Laboratory, University of New Hampshire, College of Life Sciences and Agriculture, Durham, NH, USA.
Veterinary Diagnostic Laboratory, Michigan State University, East Lansing MI, USA.
Vet Pathol. 2019 Nov;56(6):907-914. doi: 10.1177/0300985819861717. Epub 2019 Jul 22.
Five chimney swift fledglings died following a progressive loss of appetite and condition while being cared for by an experienced wildlife rehabilitator. All animals had severe necrotizing and heterophilic ventriculitis, with myriad epithelial cells characterized by karyomegaly with intranuclear inclusion bodies. Transmission electron microscopy showed distention of epithelial cell nuclei and chromatin peripheralization by nonenveloped, icosahedral, 75- to 85-nm-diameter virions. Degenerate nested PCR for a highly conserved region of the adenovirus DNA polymerase gene was positive. BLAST analysis of the amplicon sequence indicated the presence of a novel adenovirus, with 74% homology to Antarctic penguin adenoviruses and 72% homology to a bat adenovirus, at low query coverages of only 65% and 63%, respectively. BLAST analysis of the predicted amino acid sequence generated the highest scores for squamate adenoviruses at 100% query coverage. Based on phylogenetic analysis of the partial amino acid sequence of the DNA polymerase, the chimney swift virus was a novel adenovirus most closely related to the genus. Using a probe based on the novel viral sequence, DNA in situ hybridization identified viral nucleic acid in the nucleus. While the tentatively named chimney swift adenovirus-1 (CsAdV-1) is so far classified with the Atadenoviruses, it is relatively divergent from other members of that genus and may represent the first identified member of a new genus of Adenoviruses.
五只烟囱雨燕雏鸟在被一位经验丰富的野生动物康复者照顾期间，出现食欲减退和身体状况逐渐恶化的情况，最终死亡。所有动物都患有严重的坏死性和异嗜性心肌炎，大量上皮细胞表现为巨细胞症，并伴有核内包涵体。透射电子显微镜显示，上皮细胞核膨胀，染色质边缘化，由无包膜、二十面体、直径 75-85nm 的病毒粒子引起。针对腺病毒 DNA 聚合酶基因高度保守区的退化嵌套 PCR 检测结果为阳性。对扩增子序列的 BLAST 分析表明，存在一种新型腺病毒，与南极企鹅腺病毒的同源性为 74%，与蝙蝠腺病毒的同源性为 72%，查询覆盖率分别仅为 65%和 63%。对预测的氨基酸序列进行 BLAST 分析，在 100%查询覆盖率下，得分最高的是蜥蜴类腺病毒。基于 DNA 聚合酶部分氨基酸序列的系统发育分析，烟囱雨燕病毒是一种与属最密切相关的新型腺病毒。使用基于新型病毒序列的探针进行 DNA 原位杂交，可在细胞核中检测到病毒核酸。暂定名为烟囱雨燕腺病毒-1（CsAdV-1），目前被归类为 Atadenoviruses，但与该属的其他成员存在较大差异，可能代表新的腺病毒属的第一个被识别成员。
