Bagnasco M, Pesce G, Fiorino N, Riccio A M, Ciprandi G, Buscaglia S, Canonica G W
Allergy and Clinical Immunology Service, Department of Internal Medicine, University of Genoa, Italy.
Clin Exp Allergy. 1997 Jul;27(7):737-43.
The intercellular adhesion molecule ICAM-1 has been detected by immunohistochemical methods on epithelial cells of the conjunctiva and nose during allergic inflammation.
The aim of the present study was to evaluate whether ICAM-1 expression on conjunctival epithelium derives from endogenous synthesis or is merely due to passive uptake of soluble ICAM-1 released from inflammatory cells.
In situ hybridization was performed using a 3' end dygoxygenin-labelled specific DNA oligonucleotide probe on fixed conjunctival smears from allergic subjects challenged with, or naturally exposed to the allergen, and from healthy subjects. Immunocytochemistry for ICAM-1 was performed by alkaline phosphatase antialkaline phosphatase.
In allergic patients, both naturally exposed to the allergen and after specific challenge, a clear hybridization pattern on epithelial cells was apparent. Out of allergen exposure, some symptomfree pollinosic subjects, as well as a few healthy volunteers showed mild ICAM-1 mRNA cytoplasmic staining in the absence of immunohistochemically detectable ICAM-1. This finding may explain the very early appearance of ICAM-1 on conjunctival epithelium following specific challenge in allergic individuals.
These results indicate that the presence of ICAM-1 on conjunctival epithelium during allergic inflammation derives from endogenous synthesis and not from uptake of soluble ICAM-1.
在过敏性炎症期间，已通过免疫组织化学方法在结膜和鼻的上皮细胞中检测到细胞间粘附分子ICAM-1。
本研究的目的是评估结膜上皮上ICAM-1的表达是源于内源性合成还是仅仅由于炎症细胞释放的可溶性ICAM-1的被动摄取。
使用3'端地高辛标记的特异性DNA寡核苷酸探针，对来自接受过敏原激发或自然暴露于过敏原的过敏受试者以及健康受试者的固定结膜涂片进行原位杂交。通过碱性磷酸酶抗碱性磷酸酶进行ICAM-1的免疫细胞化学检测。
在自然暴露于过敏原的过敏患者以及特异性激发后，上皮细胞上出现明显的杂交模式。在未接触过敏原的情况下，一些无症状的花粉症患者以及少数健康志愿者在没有免疫组织化学可检测到的ICAM-1的情况下，显示出轻度的ICAM-1 mRNA细胞质染色。这一发现可以解释在过敏个体中特异性激发后结膜上皮上ICAM-1的非常早期的出现。
这些结果表明，过敏性炎症期间结膜上皮上ICAM-1的存在源于内源性合成，而非可溶性ICAM-1的摄取。
