Smith J E, Pasarela N R, Wyckoff J C
J Assoc Off Anal Chem. 1977 Nov;60(6):1310-7.
Polarographic residue methods have been developed for determining robenidine (Robenz), 1,3-bis[p-chlorobenzylidene)amino]-guanidine monohydrochloride, in chicken tissues, eggs, litter, soil, and plants. The compound is extracted from chicken fat, skin, muscle, liver, and eggs with ethyl acetate; from blood with acetone; from plant tissue, litter, and kidney with acidic acetone; and from soil with basic methanol. After extraction by high-speed blending or overnight shaking, the extract is cleaned up by evaporation, solvent partition and/or elution from CG-50 ion exchange resin. Robenidine is quantitated by differential cathode ray polarography, using acidic aqueous methanol or acetic acid (1+1) supporting electrolyte. Recoveries ranged from 64 to 125% with an average overall recovery of 90%. The validated sensitivity is 0.1 ppm for chicken tissues, soil, and plants, 0.01 ppm for eggs, and 1 ppm for litter.
已开发出极谱残留法用于测定鸡组织、鸡蛋、垫料、土壤和植物中的罗苯尼丁（Robenz），即1,3-双[对氯亚苄基]氨基胍盐酸盐。该化合物从鸡脂肪、皮肤、肌肉、肝脏和鸡蛋中用乙酸乙酯提取；从血液中用丙酮提取；从植物组织、垫料和肾脏中用酸性丙酮提取；从土壤中用碱性甲醇提取。通过高速搅拌或过夜振荡提取后，提取物通过蒸发、溶剂分配和/或从CG-50离子交换树脂上洗脱进行净化。罗苯尼丁通过差分阴极射线极谱法进行定量，使用酸性甲醇水溶液或乙酸（1+1）支持电解质。回收率在64%至125%之间，平均总回收率为90%。验证后的灵敏度对于鸡组织、土壤和植物为0.1 ppm，对于鸡蛋为0.01 ppm，对于垫料为1 ppm。
