产气肠杆菌中柠檬酸裂解酶的能量依赖性失活
Energy-dependent inactivation of citrate lyase in Enterobacter aerogenes.
作者信息
Kulla H, Gottschalk G
出版信息
J Bacteriol. 1977 Dec;132(3):764-70. doi: 10.1128/jb.132.3.764-770.1977.
Enterobacter aerogenes was grown in continous culture with ammonia as the growth-limiting substrate, and changes in citrate lyase and citrate synthase activities were monitored after growth shifts from anaerobic growth on citrate to aerobic growth on citrate, aerobic growth on glucose, anaerobic growth on glucose, and anaerobic growth on glucose plus nitrate. Citrate lyase was inactivated during aerobic growth on glucose and during anaerobic growth with glucose plus nitrate. Inactivation did not occur during anaerobic growth on glucose, and as a result of the simultaneous presence of citrate lyase and citrate synthase, growth difficulties were observed. Citrate lyase inactivation consisted of deacetylation of the enzyme. The corresponding deacetylase could not be demonstrated in cell extracts, and it is concluded that, as in a number of other inactivations, electron transport to oxygen or nitrate was required for inactivation.
产气肠杆菌在以氨作为生长限制底物的连续培养中生长，在从以柠檬酸盐为底物的厌氧生长转变为以柠檬酸盐为底物的需氧生长、以葡萄糖为底物的需氧生长、以葡萄糖为底物的厌氧生长以及以葡萄糖加硝酸盐为底物的厌氧生长后，监测柠檬酸裂解酶和柠檬酸合酶活性的变化。柠檬酸裂解酶在以葡萄糖为底物的需氧生长期间以及在以葡萄糖加硝酸盐为底物的厌氧生长期间失活。在以葡萄糖为底物的厌氧生长期间未发生失活，并且由于柠檬酸裂解酶和柠檬酸合酶同时存在，观察到生长困难。柠檬酸裂解酶失活包括该酶的脱乙酰化。在细胞提取物中未证实相应的脱乙酰酶，并且得出结论，与许多其他失活情况一样，失活需要向氧气或硝酸盐的电子传递。
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