Tucker V L, Victorino G P
Department of Human Physiology, School of Medicine, University of California-Devis 95612, USA.
Shock. 1997 Jul;8(1):8-15. doi: 10.1097/00024382-199707000-00003.
Ischemia-reperfusion (I/R) injury is accompanied by functional disturbances in the microcirculation that often translate into significant morbidity and mortality. We present an overview of methods to evaluate microvascular barrier properties and how these methods can be applied to the study of I/R injury. Included is a summary of techniques for measuring 1) fluid and macromolecule flux from the vascular compartment into the interstitial space of tissues and 2) the permeability parameters of microvascular wall. Several experimental models will be surveyed including whole animal, isolated organ, individually perfused microvessels and cultured endothelial cells using examples from our own studies and work of other investigators relating to I/R-induced endothelial injury. The strengths and weaknesses of these different approaches are discussed.
缺血再灌注（I/R）损伤伴随着微循环功能障碍，这常常导致显著的发病率和死亡率。我们概述了评估微血管屏障特性的方法，以及这些方法如何应用于I/R损伤的研究。内容包括测量以下指标的技术总结：1）从血管腔进入组织间隙的液体和大分子通量，以及2）微血管壁的通透性参数。将考察几种实验模型，包括全动物、离体器官、单独灌注的微血管和培养的内皮细胞，并列举我们自己的研究以及其他研究者与I/R诱导的内皮损伤相关工作的实例。讨论了这些不同方法的优缺点。
