查尔酮合酶 mRNA 的编码和 3'非编码核苷酸序列以及酶的氨基酸序列的分配。
Coding and 3' non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme.
机构信息
Biologisches Institut II der Universität, D-7800 Freiburg, FRG.
出版信息
EMBO J. 1983;2(10):1801-5. doi: 10.1002/j.1460-2075.1983.tb01661.x.
Abstract
The nucleotide sequence of an almost complete cDNA copy of chalcone synthase mRNA from cultured parsley cells (Petroselinum hortense) has been determined. The cDNA copy comprised the complete coding sequence for chalcone synthase, a short A-rich stretch of the 5' non-coding region and the complete 3' non-coding region including a poly(A) tail. The amino acid sequence deduced from the nucleotide sequence of the cDNA is consistent with a partial N-terminal sequence analysis, the total amino acid composition, the cyanogen bromide cleavage pattern, and the apparent mol. wt. of the subunit of the purified enzyme.
摘要
已测定了来自栽培欧芹细胞（Petroselinum hortense）的查尔酮合酶 mRNA 的近乎完整 cDNA 拷贝的核苷酸序列。cDNA 拷贝包含查尔酮合酶的完整编码序列、5'非编码区的短 A 丰富区以及包括聚（A）尾的完整 3'非编码区。从 cDNA 的核苷酸序列推断出的氨基酸序列与部分 N-末端序列分析、总氨基酸组成、氰化溴裂解模式以及纯化酶的亚基的表观分子量一致。
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本文引用的文献
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2
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