Noguchi E, Shibasaki M
Department of Pediatrics, Institute of Clinical Medicine, University of Tsukuba, Japan.
Int Arch Allergy Immunol. 1997 Aug;113(4):489-94. doi: 10.1159/000237626.
Using Western blotting and RAST, this study examined IgE reactivities to Dermatophagoides farinae (Df) extract in three groups of children with either asthma, atopic dermatitis (AD) or a combination of both. The Df antigen presented at least nine distinct IgE-binding bands, with apparent molecular weights of 15, 25, 30, 40, 48, 59, 67, 80 and 110 kD. Positive IgE binding reactions to 15-kD proteins were found in more than 85% of subjects in all three groups, and positive reactions to 25-, 30- and 110-kD proteins in 35-45% in each group. The frequencies of positive IgE binding reaction to other components were generally low in all three groups. The pattern of IgE reactivity to Df components did not differ significantly between the three groups of subjects. IgE-binding activities to two major allergens, Der f1 and Der f2, were also examined by RAST, using recombinant antigens. High RAST reactivities to Der f1 and Der f2 were found in three groups, and the average RAST levels for Der f1 and Der f2 did not differ significantly between the three groups. These findings suggest that a difference in IgE responsiveness to house dust mite antigen is not a major discriminative factor in determining the difference between asthma and AD.
本研究采用蛋白质免疫印迹法（Western blotting）和放射变应原吸附试验（RAST），检测了三组儿童（分别患有哮喘、特应性皮炎（AD）或两者皆有）对粉尘螨（Df）提取物的IgE反应性。Df抗原呈现出至少九条不同的IgE结合带，表观分子量分别为15、25、30、40、48、59、67、80和110 kD。在所有三组中，超过85%的受试者对15-kD蛋白有阳性IgE结合反应，每组中对25-kD、30-kD和110-kD蛋白的阳性反应率为35%-45%。在所有三组中，对其他成分的阳性IgE结合反应频率普遍较低。三组受试者对Df成分的IgE反应模式无显著差异。还使用重组抗原通过RAST检测了对两种主要变应原Der f1和Der f2的IgE结合活性。在三组中均发现对Der f1和Der f2有高RAST反应性，且三组中Der f1和Der f2的平均RAST水平无显著差异。这些发现表明，IgE对屋尘螨抗原反应性的差异不是决定哮喘和AD之间差异的主要鉴别因素。
