Kardana A, Squires M, Adam T, Goka G K, Bagshawe K D
J Immunol Methods. 1979;30(1):47-53. doi: 10.1016/0022-1759(79)90272-2.
Enzacryl polythiolactone is a cross-linked acrylamide polymer with an active thiolactone group, capable of coupling to lysine, serine and tyrosine residues. Gamma globulins from antisera specific to human chorionic gonadotropin, carcinoembryonic antigen, alpha-foetoprotein or casein were isolated using Protein A Sepharose and were subsequently coupled to enzacryl. The resulting coupled antibodies were found to provide greater sensitivity and convenience in radioimmunoassay studies than conventional double antibody precipitation methods using the same antisera.
恩扎丙烯聚硫内酯是一种带有活性硫内酯基团的交联丙烯酰胺聚合物，能够与赖氨酸、丝氨酸和酪氨酸残基偶联。使用蛋白A琼脂糖从针对人绒毛膜促性腺激素、癌胚抗原、甲胎蛋白或酪蛋白的抗血清中分离出γ球蛋白，随后将其与恩扎丙烯偶联。结果发现，与使用相同抗血清的传统双抗体沉淀法相比，所得的偶联抗体在放射免疫分析研究中具有更高的灵敏度和便利性。
