Van Heyningen V, Barron L, Brock D J, Crichton D, Lawrie S
J Immunol Methods. 1982;50(2):123-31. doi: 10.1016/0022-1759(82)90219-8.
Three mouse monoclonal antibodies directed to different epitopes on human alpha-foetoprotein have been produced. Two are of IgG1 subclass and the third is IgA. The polyethylene glycol-dependent immunoprecipitation system, designed for conventional antisera, had to be adapted before reproducible results could be obtained with the reagents. In this adapted system, as well as in a radioimmunoassay using solid-phase second antibody, a mixture of the 3 monoclonal antibodies exhibits cooperativity. However, the sensitivity of the radioimmunoassay with pooled monoclonals is not good as that of conventional antiserum. Low affinity monoclonal antibodies have been used for immunopurification of the antigen, whilst the high affinity one is useful for antigen quantitation in a labelled antibody-dependent system which requires absolute antibody specificity.
已制备出三种针对人甲胎蛋白不同表位的小鼠单克隆抗体。其中两种属于IgG1亚类，第三种是IgA。为传统抗血清设计的聚乙二醇依赖免疫沉淀系统，在使用这些试剂获得可重复结果之前必须进行调整。在这个调整后的系统中，以及在使用固相二抗的放射免疫测定中，三种单克隆抗体的混合物表现出协同作用。然而，混合单克隆抗体的放射免疫测定的灵敏度不如传统抗血清。低亲和力单克隆抗体已用于抗原的免疫纯化，而高亲和力单克隆抗体则可用于标记抗体依赖系统中的抗原定量，该系统需要绝对的抗体特异性。
