瘤胃普雷沃氏菌属模式菌株的谷氨酸脱氢酶活性谱
Glutamate dehydrogenase activity profiles for type strains of ruminal Prevotella spp.
作者信息
Wen Z, Morrison M
机构信息
Department of Animal Sciences, University of Nebraska-Lincoln 68583-0908, USA.
出版信息
Appl Environ Microbiol. 1997 Aug;63(8):3314-7. doi: 10.1128/aem.63.8.3314-3317.1997.
Abstract
The glutamate dehydrogenase (GDH) activities for the type strains of Prevotella ruminicola (strain 23), Prevotella brevis (strain GA33), and Prevotella bryantii (strain B(1)4) were assessed by a combination of enzyme assays and analysis of migration patterns of GDH proteins following nondenaturing polyacrylamide gel electrophoresis. Unlike results with most other prokaryotes, but similar to results with other members of the family Bacteroidaceae, NADPH-utilizing specific activity was greatest in all species following ammonia-limited growth. Similar also to previous findings with P. bryantii, the NAD(P)H-utilizing GDH activity of P. ruminicola can be attributed to a single protein. However, P. brevis produces an additional GDH protein(s) in response to growth with peptides. These results conclusively demonstrate that all type strains of the ruminal Prevotella sp. grouping possess GDH activity.
摘要
通过酶活性测定以及非变性聚丙烯酰胺凝胶电泳后对谷氨酸脱氢酶（GDH）蛋白迁移模式的分析，评估了瘤胃普氏菌（菌株23）、短普氏菌（菌株GA33）和布氏普氏菌（菌株B(1)4）模式菌株的谷氨酸脱氢酶（GDH）活性。与大多数其他原核生物的结果不同，但与拟杆菌科其他成员的结果相似，在氨限制生长后，所有物种利用NADPH的比活性最高。同样与之前对布氏普氏菌的研究结果相似，瘤胃普氏菌利用NAD(P)H的GDH活性可归因于单一蛋白质。然而，短普氏菌在以肽生长时会产生额外的GDH蛋白。这些结果确凿地表明，瘤胃普氏菌属的所有模式菌株都具有GDH活性。
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