高亲和力钾离子摄取系统Kdp的活性使大肠杆菌细胞对甲基乙二醛敏感。
The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal.
作者信息
Ferguson G P, Chacko A D, Lee C H, Booth I R
机构信息
Department of Molecular & Cell Biology, University of Aberdeen, United Kingdom.
出版信息
J Bacteriol. 1996 Jul;178(13):3957-61. doi: 10.1128/jb.178.13.3957-3961.1996.
Abstract
Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.
摘要
Kdp系统的表达会使细胞对甲基乙二醛（MG）敏感，无论这种亲电试剂是从外部添加还是内源性合成。这种增强的敏感性的基础是在表达Kdp的细胞中维持较高的细胞质pH值（pHi）。在这类细胞中，MG通过KefB和KefC引发细胞质快速酸化，但达到的稳态pHi仍然过高，无法提供保护。通过与乙酸孵育进一步降低pHi会增加细胞对MG的敏感性。
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