Woolley G A, Biggin P C, Schultz A, Lien L, Jaikaran D C, Breed J, Crowhurst K, Sansom M S
Department of Chemistry, University of Toronto, Canada.
Biophys J. 1997 Aug;73(2):770-8. doi: 10.1016/S0006-3495(97)78109-8.
Covalent dimers of alamethicin form conducting structures with gating properties that permit measurement of current-voltage (I-V) relationships during the lifetime of a single channel. These I-V curves demonstrate that the alamethicin channel is a rectifier that passes current preferentially, with voltages of the same sign as that of the voltage that induced opening of the channel. The degree of rectification depends on the salt concentration; single-channel I-V relationships become almost linear in 3 M potassium chloride. These properties may be qualitatively understood by using Poisson-Nernst-Planck theory and a modeled structure of the alamethicin pore.
短杆菌肽 A 的共价二聚体形成具有门控特性的导电结构，允许在单个通道的寿命期间测量电流-电压（I-V）关系。这些 I-V 曲线表明，短杆菌肽 A 通道是一个整流器，优先通过电流，其电压与诱导通道开放的电压具有相同的符号。整流程度取决于盐浓度；在 3 M 氯化钾中，单通道 I-V 关系几乎变为线性。通过使用泊松-能斯特-普朗克理论和短杆菌肽 A 孔的模拟结构，可以定性地理解这些特性。
