ω-芋螺毒素GVIA和地昔帕明不敏感的去甲肾上腺素从心脏交感神经末梢流出。

omega-Conotoxin GVIA and desipramine insensitive norepinephrine efflux from cardiac sympathetic nerve terminal.

作者信息

Yamazaki T, Kawada T, Akiyama T, Kitagawa H, Takauchi Y, Yahagi N, Sunagawa K

机构信息

Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.

出版信息

Brain Res. 1997 Jul 4;761(2):329-32. doi: 10.1016/s0006-8993(97)00443-5.

DOI:10.1016/s0006-8993(97)00443-5

PMID:9252033

Abstract

Using dialysis technique, prominent accumulation of norepinephrine (NE) in the myocardial interstitial space was observed under local administration of a monoamine oxidase inhibitor (pargyline, 10 mM), and a vesicle uptake inhibitor (reserpine, 10 microM). Accumulation of NE in the myocardial interstitial space was associated with a reduction of dihydroxyphenylglycol levels. Pretreatment with omega-conotoxin GVIA (10 microM) or desipramine (10 microM) did not suppress this response. A brisk increase in dialysate NE levels might occur as a consequence of exocytotic or neurotransporter insensitive efflux of NE.

摘要

采用透析技术，在局部给予单胺氧化酶抑制剂（优降宁，10 mM）和囊泡摄取抑制剂（利血平，10 μM）的情况下，观察到心肌间质空间中去甲肾上腺素（NE）显著蓄积。心肌间质空间中NE的蓄积与二羟苯乙二醇水平降低有关。用ω-芋螺毒素GVIA（10 μM）或地昔帕明（10 μM）预处理并未抑制这种反应。NE的胞吐作用或神经递质不敏感的外向通量可能导致透析液NE水平迅速升高。