Okumura T, Nakajima Y, Matsuoka M, Takamatsu T
Analytical Research Group, Safety and Analytical Research Center, R and D Headquarters, Shiseido Co., Ltd., Kohoku-ku, Yokohama, Japan.
J Chromatogr B Biomed Sci Appl. 1997 Jul 4;694(2):305-16. doi: 10.1016/s0378-4347(97)00106-0.
Cortisol and catecholamines are major physiological markers of human stress. In order to establish a fully automated assay system for both cortisol and catecholamines in saliva, which can be sampled without imposing stress, the previously developed system for salivary cortisol [Okumura et al., J. Chromatogr. B, 670 (1995) 11] was modified. The practical sensitivity was around 0.1 pmol ml(-1) for norepinephrine and epinephrine and 0.5 pmol ml(-1) for dopamine. The established assay procedure provided R.S.D. values of 2 approximately 3% and recoveries of 96 approximately 104% at 0.5 pmol ml(-1). Measurement of salivary catecholamines in more than 300 samples taken from about 50 healthy volunteers indicated that the normal values of norepinephrine and dopamine were very low, about 0.1 pmol ml(-1) each. In contrast to cortisol, salivary catecholamine levels did not parallel those in plasma. Nevertheless, since levels of salivary catecholamines may reflect the sympathetic nerve activity in the salivary gland, they were assayed in volunteers making a scientific presentation before a large audience. Four out of eleven volunteers reported strong feelings of fear or anxiety, and their salivary catecholamine levels were about ten times higher than normal.
皮质醇和儿茶酚胺是人类应激的主要生理标志物。为了建立一种可对唾液中的皮质醇和儿茶酚胺进行全自动检测的系统，该系统采样时不会造成应激，对之前开发的唾液皮质醇检测系统[奥村等人，《色谱杂志B》，670 (1995) 11]进行了改进。去甲肾上腺素和肾上腺素的实际检测灵敏度约为0.1 pmol/ml，多巴胺的实际检测灵敏度约为0.5 pmol/ml。既定的检测程序在0.5 pmol/ml时的相对标准偏差（R.S.D.）值为2%至3%，回收率为96%至104%。对约50名健康志愿者采集的300多个样本进行唾液儿茶酚胺检测，结果表明去甲肾上腺素和多巴胺的正常值非常低，均约为0.1 pmol/ml。与皮质醇不同，唾液儿茶酚胺水平与血浆中的水平并不平行。然而，由于唾液儿茶酚胺水平可能反映唾液腺中的交感神经活动，因此对在众多听众面前进行科学演讲的志愿者进行了检测。11名志愿者中有4名报告有强烈的恐惧或焦虑情绪，他们的唾液儿茶酚胺水平比正常水平高出约10倍。
