Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95100 Catania, Italy.
Laboratory for Molecular Surfaces and Nanotechnology-CSGI, 95125 Catania, Italy.
Molecules. 2022 Nov 3;27(21):7503. doi: 10.3390/molecules27217503.
Supramolecular recognition of dopamine by two quinoxaline cavitands was studied in solution by fluorescence titrations, ESI-MS and ROESY measurements. In addition, the tetraquinoxaline cavitand was dropped onto a siloxane-based polymeric solid support, obtaining a sensor able to detect dopamine in a linear range of concentrations 10 Mm-100 pM, with a detection limit of 1 pM, much lower than the normal concentration values in the common human fluids (plasma, urine and saliva), by using a simple smartphone as detector. This sensor shows also good selectivity for dopamine respect to the other common analytes contained in a saliva sample and can be reused after acid-base cycles, paving the way for the realization of real practical sensor for human dopamine detection.
通过荧光滴定、ESI-MS 和 ROESY 测量研究了两种喹喔啉穴醚对多巴胺的超分子识别。此外，将四喹喔啉穴醚滴加到硅氧烷基聚合物固体载体上，得到一种能够在 10 μM-100 pM 的浓度范围内检测多巴胺的传感器，检测限为 1 pM，远低于常见人体液（血浆、尿液和唾液）中的正常浓度值，使用简单的智能手机作为检测器即可实现。与唾液样本中其他常见分析物相比，该传感器对多巴胺也具有良好的选择性，并且可以在酸碱循环后重复使用，为实现用于人体多巴胺检测的实际实用传感器铺平了道路。
