Watson J
J Exp Med. 1979 Dec 1;150(6):1510-9. doi: 10.1084/jem.150.6.1510.
Murine helper T cells activated to sheep or horse erythrocyte antigens in vivo have been established as continuous cell lines in culture. T cells require the presence of a T-cell growth factor (TCGF) for continuous proliferation. TCGF purified from murine, rat, or human sources all stimulate murine T-cell growth. The T-cell mitogens concanavalin A and phytohemagglutinin do not stimulate cell proliferation in continuous T-cell lines. All cells that grow in the presence of TCGF express Thy-1 antigens. Helper activity of T-cell lines is both antigen specific and effective for syngeneic or F1 B cells. Supernates from T-cell lines do not contain antigen-specific or nonspecific helper factors. Although several T-cell lines have shown stable helper activity for greater than 50 wk in culture, other cell lines have shown a gradual decline in effector function. The procedure used to establish and maintain proliferation of T cells in culture should be suitable for the selection and growth of antigen-specific effector T cells from each subclass.
在体内被激活以针对绵羊或马红细胞抗原的小鼠辅助性T细胞，已在培养中建立为连续细胞系。T细胞持续增殖需要T细胞生长因子（TCGF）的存在。从鼠源、大鼠源或人源纯化的TCGF均能刺激小鼠T细胞生长。T细胞有丝分裂原刀豆球蛋白A和植物血凝素不会刺激连续T细胞系中的细胞增殖。在TCGF存在下生长的所有细胞均表达Thy-1抗原。T细胞系的辅助活性既是抗原特异性的，对同基因或F1 B细胞也是有效的。T细胞系的培养上清液不含抗原特异性或非特异性辅助因子。尽管有几个T细胞系在培养中已显示出超过50周的稳定辅助活性，但其他细胞系的效应功能已逐渐下降。用于在培养中建立和维持T细胞增殖的程序应适用于从每个亚类中选择和培养抗原特异性效应T细胞。
