人类抗原特异性细胞毒性T细胞系的长期培养。
Long-term culture of human antigen-specific cytotoxic T-cell lines.
作者信息
Gillis S, Baker P E, Ruscetti F W, Smith K A
出版信息
J Exp Med. 1978 Oct 1;148(4):1093-8. doi: 10.1084/jem.148.4.1093.
Abstract
Long-term cultures of human cytotoxic T-cell lines (H-CTLL) were established. H-CTLL cells were strictly dependent on growth upon a T-cell growth factor (TCGF) produced by phytohemagglutinin-stimulated normal human peripheral blood lymphocytes. H-CTLL cells were maintained in TCGF-dependent exponential proliferative culture for over 4 mo during which time they continued to mediate stimulator antigen-specific cytotoxicity as measured by a 4-h 51Cr-release assay. H-CTLL cells recovered from cryopreserved stocks and re-established in long-term culture demonstrated similar high levels of antigen-specific cytotoxicity. H-CTLL cells were 95--100% E-rosette positive and expressed normal T and Ia-like cell surface markers. The ability to sustain differentiated antigen-specific T-effector cells in long-term culture may provide a new means for the study of both the mechanism and regulation of T-cell-mediated immunity.
摘要
建立了人细胞毒性T细胞系（H-CTLL）的长期培养体系。H-CTLL细胞严格依赖由植物血凝素刺激的正常人外周血淋巴细胞产生的T细胞生长因子（TCGF）进行生长。H-CTLL细胞在依赖TCGF的指数增殖培养中维持了4个多月，在此期间，通过4小时51Cr释放试验测定，它们继续介导刺激物抗原特异性细胞毒性。从冷冻保存的细胞株中复苏并重新建立长期培养的H-CTLL细胞表现出类似的高水平抗原特异性细胞毒性。H-CTLL细胞95%-100%呈E花环阳性，并表达正常的T细胞和Ia样细胞表面标志物。在长期培养中维持分化的抗原特异性T效应细胞的能力可能为研究T细胞介导的免疫机制和调节提供一种新方法。
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