Romano G, Mancini R, Fedele P, Curigliano G, Flamini G, Giovagnoli M R, Malara N, Boninsegna A, Vecchione A, Santella R M, Cittadini A
Institute of General Pathology, Centro di Ricerche Oncologiche Giovanni XXIII, Catholic University School of Medicine, Rome, Italy.
Anticancer Res. 1997 Jul-Aug;17(4A):2827-30.
The "biologically effective dose markers", DNA and protein adducts, are a direct index of carcinogen induced cell damage and an indirect one of genetic susceptibility. This study aimed to examine the dose-response relationship for 4-Aminobiphenyl-DNA adducts in oral cells of smokers and non smokers.
An immunoperoxidase method with the monoclonal 3C8 antibody, which recognizes 4-Aminobiphenyl-DNA, has been used for detecting DNA damage in oral cells of 12 smokers and 12 non smokers.
Higher staining for 4-Aminobiphenyl-DNA was detected in the cells of smokers (187 +/- 42) vs. non smokers (135 +/- 35) (p = 0.004), with a twofold range in relative staining for both groups, suggesting individual differences relevance in metabolizing carcinogens and/or repairing DNA damage.
This non invasive method requiring small cell amounts is a tool for monitoring large groups of subjects at risk in primary prevention programs.
“生物有效剂量标志物”，即DNA和蛋白质加合物，是致癌物诱导细胞损伤的直接指标，也是遗传易感性的间接指标。本研究旨在探讨吸烟者和非吸烟者口腔细胞中4-氨基联苯-DNA加合物的剂量反应关系。
采用识别4-氨基联苯-DNA的单克隆3C8抗体的免疫过氧化物酶法，检测12名吸烟者和12名非吸烟者口腔细胞中的DNA损伤。
吸烟者细胞中4-氨基联苯-DNA的染色程度高于非吸烟者（吸烟者为187±42，非吸烟者为135±35）（p = 0.004），两组的相对染色程度有两倍的差异，表明个体在致癌物代谢和/或DNA损伤修复方面存在差异。
这种需要少量细胞的非侵入性方法是在一级预防项目中监测大量高危人群的一种工具。
