Trau D, Theueri T, Wilmer M, Meusel M, Spener F
Institute of Chemical and Biochemical Sensor Research, ICB, Münster, Germany.
Biosens Bioelectron. 1997;12(6):499-510. doi: 10.1016/s0956-5663(97)00007-9.
An amperometric flow injection immunoanalysis (FIIA) system based on an immunoreactor with immobilized biocomponents on a silica surface has been developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). In the antigen coating mode the hapten was immobilized and monoclonal primary antibody against 2,4-D together with alkaline phosphatase (AP)-labelled secondary antibody were used as sensing elements in a titration assay. In the antibody coating mode a biotinylated monoclonal antibody was immobilized on the surface of the immunoreactor and a 2,4-D-AP-conjugate was used for detection. For electrochemical measurements p-aminophenol enzymatically generated from p-aminophenyl phosphate was oxidized at a carbon working electrode at +150 mV versus Ag/AgCl. The system enabled the determination of 2,4-D in drinking water samples in the range from 0.2 to 70 micrograms/l. The whole system was computer controlled with a measuring time of 12 min for one determination.
基于在二氧化硅表面固定有生物组分的免疫反应器，开发了一种安培流动注射免疫分析（FIIA）系统，用于测定除草剂2,4-二氯苯氧乙酸（2,4-D）。在抗原包被模式下，将半抗原固定化，并将抗2,4-D单克隆一抗与碱性磷酸酶（AP）标记的二抗一起用作滴定分析中的传感元件。在抗体包被模式下，将生物素化的单克隆抗体固定在免疫反应器表面，并使用2,4-D-AP缀合物进行检测。对于电化学测量，从对氨基苯磷酸酶促生成的对氨基酚在相对于Ag/AgCl为+150 mV的碳工作电极上被氧化。该系统能够测定饮用水样品中浓度范围为0.2至70微克/升的2,4-D。整个系统由计算机控制，每次测定的测量时间为12分钟。
