Solé S, Alegret S
Grup de Sensors & Biosensors, Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Catalonia, Spain.
Environ Sci Pollut Res Int. 2001;8(4):256-64. doi: 10.1007/BF02987403.
Environmental monitoring faces the challenge of measuring an increasing number of analytes at ever decreasing concentrations. Since not all species of a given analyte have the same detrimental impact on the environment, new analytical devices and techniques are required to distinguish between the different species of a pollutant or different groups of pollutants. This paper describes analytical techniques based on biomaterials that are toxically sensitive to pollutants. This approach permits the biomonitoring of certain compounds by looking at their toxic properties. Although these techniques are based on a sound analytical strategy, their applications are limited because most of the interactions between the biological material and the analyte are irreversible. Additionally, the immobilised biological material has a limited stability. Several biomonitoring strategies based on electrochemical biosensing are discussed here and how to recover the bioactivity of biosensing system, both in discrete and automated procedures, is also reviewed.
环境监测面临着在浓度不断降低的情况下测量越来越多分析物的挑战。由于给定分析物的并非所有物种对环境都有相同的有害影响,因此需要新的分析设备和技术来区分污染物的不同物种或不同污染物组。本文描述了基于对污染物具有毒性敏感性的生物材料的分析技术。这种方法允许通过观察某些化合物的毒性特性对其进行生物监测。尽管这些技术基于合理的分析策略,但其应用受到限制,因为生物材料与分析物之间的大多数相互作用是不可逆的。此外,固定化的生物材料稳定性有限。本文讨论了几种基于电化学生物传感的生物监测策略,并回顾了如何在离散和自动化程序中恢复生物传感系统的生物活性。