Healey B G, Li L, Walt D R
Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Biosens Bioelectron. 1997;12(6):521-9. doi: 10.1016/s0956-5663(97)00009-2.
We present an optical biosensor design that expands the utility of enzyme biosensors. These biosensors are fabricated by site-selective photodeposition of analyte-sensitive polymer matrices on optical imaging fibres. These dual-analyte arrays allow for the simultaneous, independent measurement of the analyte of interest and the transducing analyte. The first integrated optical-biosensors using this design have been prepared that allow both the dependent and independent analytes to be measured simultaneously, for example penicillin and pH (Healey & Walt, 1995) or glucose and O2 (Li & Walt, 1995). Independent measurement of the transducing analyte allows penicillin or glucose to be quantitated in the presence of a concurrent pH or O2 change, respectively. Penicillin can be measured in the range 0.25-10.0 mM in the pH range 6.2-7.5. Glucose can be measured in the range 0.6-20.0 mM in the O2 range 20-100%. The utility of the sensor design was demonstrated by using the penicillin-dual-analyte biosensor to quantitate penicillin produced during a Penicillium chrysogenum fermentation.
我们展示了一种扩展酶生物传感器效用的光学生物传感器设计。这些生物传感器是通过在光学成像纤维上进行对分析物敏感的聚合物基质的位点选择性光沉积来制造的。这些双分析物阵列允许对目标分析物和传感分析物进行同时、独立的测量。已经制备了首批采用这种设计的集成光学生物传感器，它们能够同时测量相关分析物和独立分析物，例如青霉素和pH值（希利和沃尔特，1995年）或葡萄糖和氧气（李和沃尔特，1995年）。对传感分析物的独立测量使得在同时存在pH值或氧气变化的情况下，能够分别对青霉素或葡萄糖进行定量。在pH值范围为6.2 - 7.5时，青霉素的测量范围为0.25 - 10.0 mM。在氧气范围为20 - 100%时，葡萄糖的测量范围为0.6 - 20.0 mM。通过使用青霉素双分析物生物传感器对产黄青霉发酵过程中产生的青霉素进行定量，证明了该传感器设计的效用。
