Healey B G, Walt D R
Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Anal Chem. 1995 Dec 15;67(24):4471-6. doi: 10.1021/ac00120a007.
An optical penicillin biosensor is described, based on the enzyme penicillinase. The sensor is fabricated by selective photodeposition of analyte-sensitive polymer matrices on optical imaging fibers. The penicillin-sensitive matrices are fabricated by immobilizing the enzyme as micrometer-sized particles in a polymer hydrogel with a covalently bound pH indicator. An array of penicillin-sensitive and pH-sensitive matrices are fabricated on the same fiber. This array allows for the simultaneous, independent measurement of pH and penicillin. Independent measurement of the two analytes allows penicillin to be quantitated in the presence of a concurrent pH change. An analysis was conducted of enzyme kinetic parameters in order to model the penicillin response of the sensor at all pH values. This analysis accounts for the varying activity of the immobilized penicillinase at different pH values. The sensor detects penicillin in the range 0.25-10.0 mM in the pH range 6.2-7.5. The sensor was used to quantify penicillin concentration produced during a Penicillium chrysogenum fermentation.
本文描述了一种基于青霉素酶的光学青霉素生物传感器。该传感器通过在光学成像纤维上选择性光沉积对分析物敏感的聚合物基质来制备。对青霉素敏感的基质是通过将酶固定为微米级颗粒，置于含有共价结合pH指示剂的聚合物水凝胶中制成的。在同一根纤维上制备了一系列对青霉素敏感和对pH敏感的基质。该阵列允许同时独立测量pH值和青霉素。对两种分析物的独立测量使得在同时存在pH变化的情况下也能对青霉素进行定量。为了模拟传感器在所有pH值下对青霉素的响应，对酶动力学参数进行了分析。该分析考虑了固定化青霉素酶在不同pH值下活性的变化。该传感器在pH值为6.2 - 7.5的范围内可检测0.25 - 10.0 mM的青霉素。该传感器用于定量产黄青霉发酵过程中产生的青霉素浓度。
