Netuschil L, Vohrer K G, Riethe P, Kasloff Z, Brecx M
Department of Conservative Dentistry, School of Dental Medicine, University of Tübingen, FRG.
Acta Stomatol Belg. 1996 Jun;93(2):73-8.
Conflicting data continue to be presented in the literature regarding the antibacterial potential of various amalgam alloy compositions. The aim of the present study was to compare the antibacterial effects of 4 different amalgam samples on mutans streptococci using two in vitro test procedures. Glass and bovine enamel served as negative controls. The first test, one commonly used, consisted of immersing freshly prepared disks of the six materials in culture broth inoculated with mutans streptococci. Optical density measurements of the broth served to evaluate bacterial growth. This was followed by a biofilm technique which provided more intimate contact between the bacteria and specimen surfaces. With the exception of one high-copper lathe-cut amalgam, the first test revealed no antibacterial potential of the samples whereas the second test with the biofilm design elucidated significant differences in antibacterial potential between the amalgam alloy compositions and the controls. The biofilm technique, used in an in vitro test procedure, reflects the conditions of the oral environment more accurately than conventional test designs.
关于各种汞合金成分的抗菌潜力，文献中不断有相互矛盾的数据出现。本研究的目的是使用两种体外测试方法，比较4种不同汞合金样品对变形链球菌的抗菌效果。玻璃和牛牙釉质作为阴性对照。第一个测试是一种常用的方法，即将六种材料新制备的圆盘浸入接种了变形链球菌的培养液中。通过测量培养液的光密度来评估细菌生长。接下来是生物膜技术，该技术使细菌与样本表面有更密切的接触。除了一种高铜车削汞合金外，第一个测试显示样品没有抗菌潜力，而采用生物膜设计的第二个测试则表明汞合金成分与对照之间在抗菌潜力上存在显著差异。体外测试中使用的生物膜技术比传统测试设计更准确地反映了口腔环境的状况。
