Nunez L J, Schmalz G, Hembree J H
J Dent Res. 1976 Nov-Dec;55(6):1001-3. doi: 10.1177/00220345760550060101.
We will now summarize the conclusions from parts I, II and III of this study. A test procedure has been developed that provides a simple, quick, and nondestructive means of monitoring the in vitro growth of S mutans in the presence of amalgams and alloys. The spectrophotometric readings are related in a simple way to growth expressed as dry weight of bacteria and metabolic products. Results are expressed as growth relative to controls which represent bacteria growing under identical conditions but not in contact with metals. The %RA60 value that represents growth after 60 hours relative to controls is used as a measure of growth in the presence of alloys or amalgams. Spherical, fine cut, and dispersion alloys were studied as well as amalgams prepared from these alloys. The dispersion alloy inhibits growth less than the spherical alloy which in turn inhibits growth less than the fine cut alloy. The results for amalgams prepared from the alloys are reversed. At an aging time of two hours, dispersion alloy amalgams inhibit growth more than spherical alloy amalgams and fine cut alloy amalgams. Aging time of amalgams greatly influences the growth inhibition. Immediately after trituration growth is inhibited, but this inhibition is lost with aging. Different types of amalgams seem to lose growth inhibition at different rates. Hg composition in the range of 48 to 52% seems to have little effect on growth inhibition. It remains for clinical studies, which are concerned with the incidence of secondary caries associated with amalgams, to demonstrate that the age and nature of the amalgam are significant.
我们现在将总结本研究第一、二、三部分的结论。已开发出一种测试程序，该程序提供了一种简单、快速且无损的方法，用于监测变形链球菌在汞合金和合金存在下的体外生长情况。分光光度读数与以细菌和代谢产物干重表示的生长情况以一种简单的方式相关。结果表示为相对于对照组的生长情况，对照组代表在相同条件下但不与金属接触的细菌生长情况。代表60小时后相对于对照组生长情况的%RA60值用作衡量合金或汞合金存在下生长情况的指标。研究了球形、细切和弥散合金以及由这些合金制备的汞合金。弥散合金对生长的抑制作用小于球形合金，而球形合金对生长的抑制作用又小于细切合金。由这些合金制备的汞合金的结果则相反。在老化两小时时，弥散合金汞合金比球形合金汞合金和细切合金汞合金对生长的抑制作用更大。汞合金的老化时间对生长抑制有很大影响。研磨后立即生长受到抑制，但这种抑制作用会随着老化而消失。不同类型的汞合金似乎以不同的速率失去生长抑制作用。汞含量在48%至52%范围内似乎对生长抑制作用影响不大。与汞合金相关的继发龋发病率相关的临床研究仍有待证明汞合金的年龄和性质具有重要意义。
