Furugori K, Kurauchi O, Itakura A, Kanou Y, Murata Y, Mizutani S, Seo H, Tomoda Y, Nakamura T
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Nagoya, Japan.
J Clin Endocrinol Metab. 1997 Aug;82(8):2726-30. doi: 10.1210/jcem.82.8.4176.
The purpose of this study was to elucidate the possible relationship between hepatocyte growth factor (HGF) expression and the pathogenesis of preeclampsia. The concentration of immunoreactive HGF was measured and the expression of HGF messenger ribonucleic acid (mRNA) assessed in human placentas obtained from two groups: uncomplicated and preeclamptic pregnancies at various gestational weeks. In addition, the localization of HGF mRNA and c-met protein was analyzed using in situ hybridization and immunohistochemical staining, respectively. The expression of HGF mRNA and the concentration of immunoreactive HGF were highest in second trimester and were significantly decreased in preeclamptic placentas compared with the uncomplicated cases in third trimester. HGF mRNA was localized to placental mesenchymal cells, whereas c-met protein was demonstrated on cytotrophoblast. These results provide evidence of an abnormality of HGF expression in the preeclamptic placentas. Such placentas exhibit the abnormally shallow trophoblast invasion of the uterus, and reduced expression of HGF could well account for this morphometric change.
本研究的目的是阐明肝细胞生长因子（HGF）表达与子痫前期发病机制之间的可能关系。测定了免疫反应性HGF的浓度，并评估了从两组获得的人胎盘中HGF信使核糖核酸（mRNA）的表达：不同孕周的正常妊娠和子痫前期妊娠。此外，分别使用原位杂交和免疫组织化学染色分析了HGF mRNA和c-met蛋白的定位。HGF mRNA的表达和免疫反应性HGF的浓度在妊娠中期最高，与妊娠晚期的正常病例相比，子痫前期胎盘显著降低。HGF mRNA定位于胎盘间充质细胞，而c-met蛋白在细胞滋养层上表达。这些结果提供了子痫前期胎盘HGF表达异常的证据。此类胎盘表现出滋养层对子宫的侵袭异常浅，而HGF表达降低很可能解释了这种形态学变化。
