Chawla M K, Gutierrez G M, Young W S, McMullen N T, Rance N E
Department of Cell Biology and Anatomy, University of Arizona College of Medicine, Tucson 85724, USA.
J Comp Neurol. 1997 Aug 4;384(3):429-42. doi: 10.1002/(sici)1096-9861(19970804)384:3<429::aid-cne8>3.0.co;2-5.
In situ hybridization histochemistry was used to map the distribution of neurons expressing the substance P (SP) or neurokinin B (NKB) genes in the human hypothalamus and basal forebrain. Hypothalami from five adult males were frozen in isopentane at -30 degrees C and serially sectioned at 20 jm thickness. Every 20th section was hybridized with [35S]-labeled, 48-base synthetic cDNA probes that were complementary to either SP or NKB mRNAs. Slides were dipped into nuclear emulsion for visualization of mRNAs at the single-cell level. The location of labeled neurons (greater than x 5 background) was mapped by using an image-combining computer microscope system. A distinct and complementary distribution pattern of SP and NKB neurons was observed in the human hypothalamus and basal forebrain. NKB was the predominant tachykinin in the rostral hypothalamus, whereas SP mRNA predominated in the posterior hypothalamus. Numerous NKB neurons were identified in the magnocellular basal forebrain, the bed nucleus of stria terminalis, and the anterior hypothalamic area. Scattered NKB neurons were present in the infundibular and paraventricular nuclei, paraolfactory gyrus, posterior hypothalamic area, lateral division of the medial mammillary nucleus, and amygdala. Numerous neurons expressing SP mRNAs were identified in the premammillary, supramammillary, and medial mammillary nuclei; the posterior hypothalamic area; and the corpus striatum. Scattered SP neurons were also observed in the preoptic area; the infundibular, intermediate, dorsomedial, and ventromedial nuclei; the infundibular stalk; the amygdala; the bed nucleus of stria terminalis; and the paraolfactory gyrus. These studies provide the first description of the location of neurons that express tachykinin gene transcripts in the human hypothalamus.
采用原位杂交组织化学方法绘制了表达P物质（SP）或神经激肽B（NKB）基因的神经元在人类下丘脑和基底前脑的分布图。取5名成年男性的下丘脑，在-30℃的异戊烷中冷冻，然后连续切片，厚度为20μm。每隔20张切片与[35S]标记的、48个碱基的合成cDNA探针杂交，该探针与SP或NKB mRNA互补。将玻片浸入核乳剂中，以便在单细胞水平上观察mRNA。使用图像合成计算机显微镜系统绘制标记神经元（大于背景值5倍）的位置。在人类下丘脑和基底前脑中观察到SP和NKB神经元有明显且互补的分布模式。NKB是下丘脑前部主要的速激肽，而SP mRNA在下丘脑后部占主导。在大细胞基底前脑、终纹床核和下丘脑前区发现了大量NKB神经元。在漏斗核和室旁核、旁嗅回、下丘脑后区、内侧乳头核外侧部和杏仁核中存在散在的NKB神经元。在乳头前核、乳头体上核和内侧乳头核、下丘脑后区以及纹状体中发现了大量表达SP mRNA的神经元。在视前区、漏斗核、中间核、背内侧核和腹内侧核、漏斗柄、杏仁核、终纹床核和旁嗅回中也观察到散在的SP神经元。这些研究首次描述了人类下丘脑中表达速激肽基因转录本的神经元的位置。
