Darlington D N, Mains R E, Eipper B A
Department of Surgery, University of Maryland School of Medicine, Baltimore 21201, USA.
Neuroscience. 1996 Mar;71(2):477-88. doi: 10.1016/0306-4522(95)00458-0.
In situ hybridization for regulated endocrine-specific protein-18 messenger RNA showed a distinct and limited pattern of expression in the hypothalamus, midline thalamus, amygdala and hippocampus of the rat. High levels of regulated endocrine-specific protein-18 messenger RNA were found in the magnocellular neurons of the hypothalamic paraventricular, supraoptic and accessory nuclei, in the neurons of the periventricular, medial tuberal, arcuate, lateral and perifornical nuclei, infundibular stalk, and in the ventrolateral division of the ventromedial nucleus and compact division of the dorsomedial nucleus. Lower levels of regulated endocrine-specific protein-18 messenger RNA were found in the parvocellular divisions of the paraventricular nucleus as well as in the bed nucleus of the stria terminalis, median preoptic nucleus, medial preoptic nucleus, medial and lateral preoptic areas, subfornical organ, suprachiasmatic nucleus, anterior hypothalamic area, zona incerta, ventromedial nucleus, dorsomedial nucleus and tuber cinereum. Regulated endocrine-specific protein-18 messenger RNA was also found in thalamic structures including the paraventricular, central medial, intermediodorsal, anterodorsal, rhomboid and reticular nuclei. Signal was also identified in the medial and lateral habenula, in the central, medial, basomedial and anterior cortical nuclei of the amygdala, and in the CA1-CA3 and dentate gyrus of the hippocampus. Dopamine may regulate regulated endocrine-specific protein-18 expression in the CNS because (i) regulated endocrine-specific protein-18 was originally identified in melanotropes based on its regulation by dopaminergic agents and (ii) many of the nuclei that contain regulated endocrine-specific protein-18 also receive dopaminergic input. The localization of regulated endocrine-specific protein-18 in the diencephalon suggests that regulated endocrine-specific protein-18 is involved in regulation of limbic and autonomic function, neuroendocrine control of salt and water balance, reproductive function and feeding behavior.
原位杂交检测受调控的内分泌特异性蛋白-18信使核糖核酸显示，在大鼠下丘脑、丘脑中线、杏仁核和海马中存在独特且有限的表达模式。在下丘脑室旁核、视上核和副核的大细胞神经元中，室周核、内侧结节核、弓状核、外侧核和穹窿周核、漏斗柄的神经元中，以及腹内侧核的腹外侧部和背内侧核的致密部中发现了高水平的受调控的内分泌特异性蛋白-18信使核糖核酸。在室旁核的小细胞部以及终纹床核、视前正中核、视前内侧核、视前内侧和外侧区、穹窿下器官、视交叉上核、下丘脑前区、未定带、腹内侧核、背内侧核和灰结节中发现了较低水平的受调控的内分泌特异性蛋白-18信使核糖核酸。在包括室旁核、中央内侧核、中间背核、前背核、菱形核和网状核在内的丘脑结构中也发现了受调控的内分泌特异性蛋白-18信使核糖核酸。在内侧和外侧缰核、杏仁核的中央、内侧、基底内侧和前皮质核以及海马的CA1-CA3和齿状回中也鉴定到了信号。多巴胺可能调节中枢神经系统中受调控的内分泌特异性蛋白-18的表达，因为（i）受调控的内分泌特异性蛋白-18最初是在促黑素细胞中基于其受多巴胺能药物的调控而被鉴定出来的，并且（ii）许多含有受调控的内分泌特异性蛋白-18的核也接受多巴胺能输入。受调控的内分泌特异性蛋白-18在间脑中的定位表明，受调控的内分泌特异性蛋白-18参与边缘和自主功能的调节、盐和水平衡的神经内分泌控制、生殖功能和摄食行为。
