Sce3是粟酒裂殖酵母隔膜突变体cdc11的一个抑制因子，编码一种假定的RNA结合蛋白。
Sce3, a suppressor of the Schizosaccharomyces pombe septation mutant cdc11, encodes a putative RNA-binding protein.
作者信息
Schmidt S, Hofmann K, Simanis V
机构信息
Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), Chemin des Boveresses 155, 1066 Epalinges, Switzerland.
出版信息
Nucleic Acids Res. 1997 Sep 1;25(17):3433-9. doi: 10.1093/nar/25.17.3433.
Abstract
In the fission yeast Schizosaccharomyces pombe, the cdc11 gene is required for the initiation of septum formation at the end of mitosis. The sce3 gene was cloned as a multi-copy suppressor of the heat-sensitive mutant cdc11-136. When over-expressed, it rescues all mutants of cdc11 and also a heat-sensitive allele of cdc14, but not the cdc14 null mutant. Deletion shows that sce3 is not essential for cell proliferation. It encodes a putative RNA-binding protein which shows homology to human eIF4B. Immunolocalisation indicates that Sce3p is located predominantly in the cytoplasm. Elevated expression of sce3 increases the steady-state level of cdc14 mRNA. Possible mechanisms of its action are discussed.
摘要
在裂殖酵母粟酒裂殖酵母中，cdc11基因是有丝分裂末期隔膜形成起始所必需的。sce3基因作为热敏感突变体cdc11 - 136的多拷贝抑制子被克隆出来。当过度表达时，它能拯救所有cdc11突变体以及cdc14的一个热敏感等位基因，但不能拯救cdc14缺失突变体。缺失实验表明sce3对细胞增殖不是必需的。它编码一种推定的RNA结合蛋白，该蛋白与人类eIF4B具有同源性。免疫定位表明Sce3p主要位于细胞质中。sce3的表达升高会增加cdc14 mRNA的稳态水平。文中讨论了其可能的作用机制。
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Genes Dev. 1997 Jun 15;11(12):1519-34. doi: 10.1101/gad.11.12.1519.
2
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J Cell Biol. 1997 Apr 7;137(1):169-82. doi: 10.1083/jcb.137.1.169.
3
Requirement of the DEAD-Box protein ded1p for messenger RNA translation.DEAD盒蛋白ded1p对信使核糖核酸翻译的需求。
Science. 1997 Mar 7;275(5305):1468-71. doi: 10.1126/science.275.5305.1468.
4
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Trends Genet. 1996 May;12(5):171-5. doi: 10.1016/0168-9525(96)10016-0.
5
6
7
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