Kawahara Y, Takahashi-Fuke K, Shimizu E, Nakamatsu T, Nakamori S
Technology Laboratory of Ajinomoto Co., Inc., Kawasaki, Japan.
Biosci Biotechnol Biochem. 1997 Jul;61(7):1109-12. doi: 10.1271/bbb.61.1109.
Enzyme activities of 2-oxoglutarate dehydrogenase complex and glutamate dehydrogenase of wild type Brevibacterium lactofermentum, one of the typical glutamate-producing coryneform bacteria, were investigated by using cells cultured under glutamate-productive and glutamate-non-productive conditions. Significant reduction of the former enzyme activity was observed in the cells under the several glutamate-productive conditions, namely, in the cells cultured in media containing a) limited concentrations of biotin, b) sub-lethal amounts of penicillin, and c) sub-optimal amounts of a surface-active agent, as compared with those under the non-productive conditions. The activity of the latter enzyme was essentially unchanged in every condition. The relationship between glutamate production and the enzyme activities as well as permeability of glutamate through cell membrane was discussed from the results obtained.
利用在谷氨酸生产条件和非生产条件下培养的细胞，对典型的谷氨酸生产棒状杆菌之一的野生型乳酸发酵短杆菌的2-氧代戊二酸脱氢酶复合体和谷氨酸脱氢酶的酶活性进行了研究。与非生产条件下的细胞相比，在几种谷氨酸生产条件下，即在含有a）有限浓度生物素、b）亚致死量青霉素和c）次最佳量表面活性剂的培养基中培养的细胞中，观察到前一种酶活性显著降低。后一种酶的活性在每种条件下基本保持不变。根据所得结果，讨论了谷氨酸生产与酶活性以及谷氨酸透过细胞膜的通透性之间的关系。
