Ko Y T, Chipley J R
Microbios. 1984;40(161-162):161-71.
To investigate the role of biotin in lysine production, Brevibacterium lactofermentum ATCC 21086 was grown in an acid-hydrolysed whey permeate medium with and without added biotin. Added biotin stimulated lysine production and growth of B. lactofermentum. Five micrograms of biotin/100 ml was the optimum level of addition. Biotin increased the uptake of 14C-glucose and affected fatty acid composition of cell wall lipids. Cell walls of test organisms contained less 16:0 and more 18:2 fatty acids than did those from control cells. However, biotin did not substantially affect the phospholipid content of cell walls and whole cells, and the intracellular free lysine level. It was concluded that the promotive effect of biotin in lysine production might be due to the stimulatory effect of biotin on the growth of B. lactofermentum. Alteration of the cell surface caused by biotin did not appear to affect the release or accumulation of lysine.
为研究生物素在赖氨酸生产中的作用，将乳酸发酵短杆菌ATCC 21086在添加和不添加生物素的酸水解乳清渗透液培养基中培养。添加生物素可刺激乳酸发酵短杆菌的赖氨酸生产和生长。每100毫升添加5微克生物素为最佳添加水平。生物素增加了14C-葡萄糖的摄取，并影响细胞壁脂质的脂肪酸组成。与对照细胞相比，受试生物的细胞壁含有的16:0脂肪酸较少，18:2脂肪酸较多。然而，生物素对细胞壁和全细胞的磷脂含量以及细胞内游离赖氨酸水平没有实质性影响。得出的结论是，生物素在赖氨酸生产中的促进作用可能是由于生物素对乳酸发酵短杆菌生长的刺激作用。生物素引起的细胞表面变化似乎并未影响赖氨酸的释放或积累。
