环糊精葡糖基转移酶通过转糖基化反应合成糖基海藻糖。

Synthesis of glycosyl-trehaloses by cyclomaltodextrin glucanotransferase through the transglycosylation reaction.

作者信息

Kurimoto M, Tabuchi A, Mandai T, Shibuya T, Chaen H, Fukuda S, Sugimoto T, Tsujisaka Y

机构信息

Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.

出版信息

Biosci Biotechnol Biochem. 1997 Jul;61(7):1146-9. doi: 10.1271/bbb.61.1146.

DOI:10.1271/bbb.61.1146

PMID:9255978

Abstract

Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of glycosyl-trehaloses through the transglycosylation reaction with cyclomaltohexaose as the glycosyl donor and trehalose as its acceptor. After beta-amylase treatment, five species of glycosyl-trehaloses were isolated by column chromatography. After chemical and enzymatic analyses, it was concluded that these oligosaccharides were alpha-maltosyl alpha-D-glucopyranoside, alpha-maltotriosyl alpha-D-glucopyranoside, alpha-maltosyl alpha-maltoside, alpha-maltotriosyl alpha-maltoside, and alpha-maltotriosyl alpha-maltotrioside. These were not hydrolyzed by salivary amylase, artificial gastric juice, or pancreatic amylase, however they were hydrolyzed by enzymes of the small intestine.

摘要

嗜热脂肪芽孢杆菌的环麦芽糊精葡糖基转移酶以环麦芽六糖作为糖基供体、海藻糖作为受体，通过转糖基化反应生成了一系列糖基海藻糖。经β-淀粉酶处理后，通过柱色谱法分离出了五种糖基海藻糖。经过化学和酶学分析，得出这些寡糖分别是α-麦芽糖基α-D-吡喃葡萄糖苷、α-麦芽三糖基α-D-吡喃葡萄糖苷、α-麦芽糖基α-麦芽糖苷、α-麦芽三糖基α-麦芽糖苷和α-麦芽三糖基α-麦芽三糖苷。这些寡糖不会被唾液淀粉酶、人工胃液或胰淀粉酶水解，然而它们会被小肠中的酶水解。