Ogawa N, Ohashi H
Third Department of Internal Medicine, Hamamatsu University School of Medicine.
Rinsho Byori. 1997 Jul;45(7):643-8.
To clarify the role of apoptosis in the pathogenesis of Sjögren's syndrome (SS), we investigated apoptosis and apoptosis-related molecules in peripheral blood lymphocytes (PBL) and salivary glands (SG) from SS patients and normal controls. In PBL, SS T lymphocytes showed accelerated in vitro apoptosis, and CD4+ T cells showed increased Fas expression compared to those in normal controls. Interestingly, SS T cells also showed increased Bcl-2 expression. The acinar epithelial cells in SS were Fas+ and FasL+, and these cells died by apoptosis. The majority of infiltrating lymphocytes in SS were Fas+ and Bcl-2+, while few lymphocytes expressed FasL. In situ detection of apoptosis showed minimal cell death among lymphocytes, particularly in dense periductal foci. Bax seemed to be expressed in apoptotic acinar cells. In conclusion, blocked apoptosis of SG infiltrating lymphocytes as well as enhanced apoptosis of peripheral T cells may be characteristics of SS. SS acinar cells may die by apoptosis, and this may ultimately lead to SG destruction in SS patients.
为阐明细胞凋亡在干燥综合征（SS）发病机制中的作用，我们研究了SS患者和正常对照者外周血淋巴细胞（PBL）及唾液腺（SG）中的细胞凋亡及凋亡相关分子。在PBL中，SS T淋巴细胞体外凋亡加速，与正常对照相比，CD4⁺ T细胞Fas表达增加。有趣的是，SS T细胞Bcl-2表达也增加。SS中的腺泡上皮细胞Fas⁺且FasL⁺，这些细胞通过凋亡死亡。SS中大多数浸润淋巴细胞Fas⁺且Bcl-2⁺，而很少有淋巴细胞表达FasL。细胞凋亡的原位检测显示淋巴细胞中细胞死亡极少，尤其是在密集的导管周围病灶处。Bax似乎在凋亡的腺泡细胞中表达。总之，SG浸润淋巴细胞凋亡受阻以及外周T细胞凋亡增强可能是SS的特征。SS腺泡细胞可能通过凋亡死亡，这最终可能导致SS患者的SG破坏。
