Johnston J P, Farhangfar F, Aparicio J G, Nam S H, Applebury M L
Department of Ophthalmology, Harvard Medical School/MEEI, Boston, MA 02114, USA.
Gene. 1997 Jul 9;193(2):219-27. doi: 10.1016/s0378-1119(97)00125-x.
The gene encoding the bovine guanylate cyclase isoform E (GC-E) was isolated as a single 18 kb genomic clone and shown to have 20 exons and 19 introns. Comparison of the structure of the GC-E gene with structures of other membrane guanylate cyclase genes indicates that the GC-E is most closely related to the subfamily of sensory guanylate cyclases. Comparison of the GC-E structure with that of the more distantly related guanylate cyclase isoform A (GC-A) gene shows the most divergence in the extracellular and C-terminal regions, but general conservation of introns and exons in the intracellular kinase-like and catalytic domains. RT-PCR from several bovine tissues shows that GC-E is expressed only in the retina. Consistent with this pattern of expression, elements for the retinal-specific transcription factors RET-1, RET-2 and Talpha-1 are located in the 5' flanking promoter region.
编码牛鸟苷酸环化酶同工型E（GC-E）的基因作为一个单一的18 kb基因组克隆被分离出来，显示有20个外显子和19个内含子。将GC-E基因的结构与其他膜鸟苷酸环化酶基因的结构进行比较表明，GC-E与感觉鸟苷酸环化酶亚家族关系最为密切。将GC-E的结构与关系更远的鸟苷酸环化酶同工型A（GC-A）基因的结构进行比较，结果显示在细胞外和C末端区域差异最大，但在细胞内激酶样结构域和催化结构域中外显子和内含子总体保守。对多个牛组织进行的逆转录聚合酶链反应（RT-PCR）表明，GC-E仅在视网膜中表达。与这种表达模式一致，视网膜特异性转录因子RET-1、RET-2和Tα-1的元件位于5'侧翼启动子区域。
