Inoue H, Ohshima H, Kono H, Yamanaka M, Kubota T, Aihara M, Hiroi T, Yago N, Ishida H
Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
Biochem Pharmacol. 1997 Jun 15;53(12):1941-4. doi: 10.1016/s0006-2952(97)00187-1.
We investigated the effects of tranilast on inducible cyclooxygenase (COX2)-mediated prostaglandin E2 (PGE2) production and enzyme induction in interleukin-lbeta (IL-1beta)-stimulated cultured dermal fibroblasts. IL-1beta enhanced PGE2 production in cultured fibroblasts. Tranilast did not affect constitutive cyclooxygenase (COX1) or COX2 activity in non-stimulated or IL-lbeta-stimulated fibroblasts. However, the COX2 expression induced by IL-1beta was inhibited by tranilast. This result, that IL-1beta-induced COX2 expression was suppressed by tranilast, was confirmed by immunohistochemical analysis. Thus, it is possible for tranilast to regulate PGE2 production by inhibiting COX2 induction.
我们研究了曲尼司特对白细胞介素-1β（IL-1β）刺激的培养皮肤成纤维细胞中诱导型环氧化酶（COX2）介导的前列腺素E2（PGE2）产生及酶诱导的影响。IL-1β增强了培养成纤维细胞中PGE2的产生。曲尼司特对未刺激或IL-1β刺激的成纤维细胞中的组成型环氧化酶（COX1）或COX2活性没有影响。然而，曲尼司特抑制了IL-1β诱导的COX2表达。免疫组织化学分析证实了曲尼司特抑制IL-1β诱导的COX2表达这一结果。因此，曲尼司特有可能通过抑制COX2诱导来调节PGE2的产生。
