真核生物翻译起始因子介导的mRNA环化
Circularization of mRNA by eukaryotic translation initiation factors.
作者信息
Wells S E, Hillner P E, Vale R D, Sachs A B
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
出版信息
Mol Cell. 1998 Jul;2(1):135-40. doi: 10.1016/s1097-2765(00)80122-7.
Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor eIF4G. Here, we demonstrate the reconstitution of an eIF4E/eIF4G/Pab1p complex with recombinant proteins, and show by atomic force microscopy that the complex can circularize capped, polyadenylated RNA. Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell.
真核生物信使核糖核酸（mRNA）的5' 帽结构与3' 聚腺苷酸（poly(A)）尾之间的通讯导致翻译的协同增强。帽结合蛋白和聚腺苷酸尾结合蛋白，即真核起始因子4E（eIF4E）和聚腺苷酸结合蛋白1（Pab1p），在酿酒酵母中通过它们与翻译因子eIF4G不同部分的相互作用介导这种效应。在这里，我们展示了用重组蛋白重建eIF4E/eIF4G/Pab1p复合物，并通过原子力显微镜表明该复合物可以使带帽、聚腺苷酸化的RNA环化。我们的结果表明，翻译因子形成环状mRNA可能有助于真核细胞中mRNA表达的控制。
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