Gamblin R M, Sagartz J E, Couto C G
Department of Veterinary Clinical Sciences, Ohio State University, Columbus 43210, USA.
Am J Vet Res. 1997 Aug;58(8):857-63.
To determine prevalence of p53 tumor suppressor protein overexpression in spontaneously arising tumors of dogs, using the CM-1 polyclonal antibody and immunohistochemical methods. DESIGN AND SAMPLE POPULATION: Retrospective analysis was performed on archived, paraffin-embedded tumor tissue from dogs. A total of 226 tumors were evaluated, including tumors of epithelial, mesenchymal, and round cell origins.
Overexpression of p53 was detected by indirect immunohistochemical methods, using the CM-1 rabbit anti-human p53 polyclonal primary antibody. Protein overexpression was determined by use of a grading system based on percentage of stained tumor nuclei.
Nuclear overexpression of p53 was detected in most squamous cell carcinomas, nasal adenocarcinomas, and perianal gland adenocarcinomas. Hemangiopericytomas, transitional cell carcinomas, mammary adenocarcinomas, apocrine gland adenocarcinomas, intestinal adenocarcinomas, mast cell tumors, and cutaneous histiocytomas had low numbers of nuclei overexpressing p53. Remaining tumor types had intermediate p53 nuclear overexpression. Cytoplasmic staining was observed in some carcinomas, particularly intestinal adenocarcinomas.
Overexpression of p53 is common in spontaneously arising neoplasms of dogs.
Prospective determination of p53 status in some tumor types may be as clinically useful in determining prognosis and predicting survival times for dogs with cancer as it is for human beings with cancer.
使用CM-1多克隆抗体和免疫组织化学方法，确定犬自发性肿瘤中p53肿瘤抑制蛋白过表达的患病率。
对存档的犬石蜡包埋肿瘤组织进行回顾性分析。共评估了226个肿瘤，包括上皮、间充质和圆形细胞起源的肿瘤。
采用间接免疫组织化学方法，使用CM-1兔抗人p53多克隆一抗检测p53的过表达。通过基于染色肿瘤细胞核百分比评分系统确定蛋白过表达情况。
在大多数鳞状细胞癌、鼻腺癌和肛周腺腺癌中检测到p53核过表达。血管外皮细胞瘤、移行细胞癌、乳腺腺癌、顶泌汗腺腺癌、肠腺癌、肥大细胞瘤和皮肤组织细胞瘤中p53过表达的细胞核数量较少。其余肿瘤类型的p53核过表达情况中等。在一些癌中观察到细胞质染色，尤其是肠腺癌。
p53过表达在犬自发性肿瘤中很常见。
对某些肿瘤类型进行p53状态的前瞻性测定，在确定犬癌症预后和预测生存时间方面可能与人类癌症一样具有临床实用性。
