Jaffe M H, Hosgood G, Taylor H W, Kerwin S C, Hedlund C S, Lopez M K, Davidson J R, Miller D M, Paranjpe M
Department of Veterinary Clinical Science, Louisiana State University School of Veterinary Medicine, Baton Rouge 70803, USA.
Vet Pathol. 2000 Jan;37(1):40-6. doi: 10.1354/vp.37-1-40.
One hundred twenty-six cutaneous mast cell tumors obtained by excisional biopsy from 106 dogs were evaluated using immunohistochemical staining for the presence of p53 protein. A standard avidin-biotin immunohistochemical protocol was used incorporating a polyclonal antibody of rabbit origin (CM-1) as the primary antibody. Histopathologic grading of tumors was performed on hemotoxylin and eosin-stained samples. There was a significant difference in the percentage of cells staining positive for p53 for the histopathologic grades (P = 0.0005). Grade III tumors had a significantly greater p53 content than did grade I or II tumors (P < 0.05). Clinical data obtained retrospectively was available for 54 dogs. Tumor recurred in 19 of 54 (35.2%) dogs. Twenty-nine dogs died by the end of the study; 9 of 29 (31.0%) died of mast cell tumor disease. Histopathologic grade showed a significant negative association with survival time. Both clinical stage and histopathologic grade showed a significant negative association with time to recurrence. The percentage of cells staining positive for p53 did not significantly improve the forward analysis. Immunohistochemical detection of p53 did not appear useful in characterizing the clinical association between cutaneous mast cell tumor cellular features and survival time or time to tumor recurrence in dogs.
对106只犬经切除活检获得的126个皮肤肥大细胞瘤，采用免疫组织化学染色法评估p53蛋白的表达情况。采用标准抗生物素蛋白-生物素免疫组织化学方法，以兔源多克隆抗体（CM-1）作为一抗。对苏木精和伊红染色的样本进行肿瘤组织病理学分级。p53染色阳性细胞的百分比在组织病理学分级上存在显著差异（P = 0.0005）。III级肿瘤的p53含量显著高于I级或II级肿瘤（P < 0.05）。对54只犬进行了回顾性临床数据收集。54只犬中有19只（35.2%）肿瘤复发。到研究结束时，29只犬死亡；29只中有9只（31.0%）死于肥大细胞瘤疾病。组织病理学分级与生存时间呈显著负相关。临床分期和组织病理学分级与肿瘤复发时间均呈显著负相关。p53染色阳性细胞的百分比在正向分析中未显示出显著改善。免疫组织化学检测p53在描述犬皮肤肥大细胞瘤细胞特征与生存时间或肿瘤复发时间之间的临床关联方面似乎并无用处。
