Daheshia M, Kuklin N, Kanangat S, Manickan E, Rouse B T
Department of Microbiology, The University of Tennessee, Knoxville 37996, USA.
J Immunol. 1997 Aug 15;159(4):1945-52.
Ocular infection with herpes simplex virus leads to an inflammatory lesion in the cornea orchestrated by CD4+ Th1 lymphocytes. This immunopathologic disease, called herpetic stromal keratitis, is an important cause of impaired vision. In this study, we set out to determine whether established lesions of herpetic stromal keratitis could be controlled by topically administering naked plasmid DNA encoding cytokines to the corneal surface. A single topical administration of DNA encoding IL-10 was beneficial to the majority (75%) of treated animals, and 50% (vs 10% in controls) resolved their lesions completely over a 23-day observation period. Topical ocular application of DNA encoding foreign proteins was also shown to be an effective means of inducing systemic and mucosal immune responses. The direct application of DNA encoding cytokines may represent an additional therapeutic option for the management of immunoinflammatory disease.
单纯疱疹病毒引起的眼部感染会导致角膜出现由CD4 + Th1淋巴细胞介导的炎性病变。这种免疫病理疾病称为疱疹性基质性角膜炎，是视力受损的一个重要原因。在本研究中，我们着手确定通过向角膜表面局部施用编码细胞因子的裸质粒DNA，是否能够控制已形成的疱疹性基质性角膜炎病变。单次局部施用编码IL-10的DNA对大多数（75%）受试动物有益，并且在23天的观察期内，50%（对照组为10%）的动物病变完全消退。向眼部局部应用编码外源蛋白的DNA也被证明是诱导全身和黏膜免疫反应的有效手段。直接应用编码细胞因子的DNA可能为免疫炎性疾病的治疗提供一种额外的选择。
