Palmer J S, Cromie W J, Plzak L F, Leff A R
Section of Urology, University of Chicago Pritzker School of Medicine, Illinois, USA.
J Urol. 1997 Sep;158(3 Pt 2):1186-90. doi: 10.1097/00005392-199709000-00128.
Platelet activating factor, a biochemical marker and lipid mediator of ischemic injury, has been demonstrated in several organ systems. The objective of this study was to investigate the possible role of platelet activating factor in testicular ischemic injury.
Five groups of 6 male Sprague-Dawley rats were studied, including group 1-nonoperated controls, group 2-sham operated controls, group 3-those that underwent administration of 10 micrograms./kg. exogenous platelet activating factor into the left testicular artery, group 4-those that underwent 4 hours of testicular ischemia and group 5-those that received pretreatment with 0.4 mg./kg. of the platelet activating factor antagonist CV-6209 intravenously before 4 hours of testicular ischemia. Ipsilateral and contralateral testes were examined histologically and seminiferous tubular diameters were measured.
Exogenous platelet activating factor administration in group 3 and 4 hours of ischemia in group 4 resulted in a similar extent of histological degeneration of the experimental testicle. Pretreatment with CV-6209 in group 5 resulted in a marked decrease in hemorrhagic discoloration, vascular congestion and histological changes noted with ischemia in group 4.
The results of this study suggest that platelet activating factor has a biochemical role in tissue injury associated with testicular ischemia. Also, administration of a platelet activating factor antagonist before the ischemic event decreases seminiferous tubule degeneration.
血小板活化因子是缺血性损伤的一种生化标志物和脂质介质，已在多个器官系统中得到证实。本研究的目的是探讨血小板活化因子在睾丸缺血性损伤中的可能作用。
对五组每组6只雄性Sprague-Dawley大鼠进行研究，包括第1组——未手术对照组，第2组——假手术对照组，第3组——经左睾丸动脉注射10微克/千克外源性血小板活化因子的大鼠，第4组——经历4小时睾丸缺血的大鼠，以及第5组——在睾丸缺血4小时前静脉注射0.4毫克/千克血小板活化因子拮抗剂CV-6209进行预处理的大鼠。对同侧和对侧睾丸进行组织学检查并测量生精小管直径。
第3组给予外源性血小板活化因子以及第4组缺血4小时后，实验侧睾丸的组织学退变程度相似。第5组用CV-6209预处理后，出血性变色、血管充血以及第4组缺血时出现的组织学变化均明显减轻。
本研究结果表明，血小板活化因子在与睾丸缺血相关的组织损伤中具有生化作用。此外，在缺血事件发生前给予血小板活化因子拮抗剂可减少生精小管退变。
