Marques-Vidal P, Jauhiainen M, Metso J, Ehnholm C
National Public Health Institute, Department of Biochemistry, Helsinki, Finland.
Atherosclerosis. 1997 Aug;133(1):87-95. doi: 10.1016/s0021-9150(97)00120-2.
High density lipoprotein 2 (HDL2) was incubated with phospholipid transfer protein (PLTP) or with hepatic lipase (H-TGL), and the incubation products were separated into a d < 1.22 g/ml and a d > 1.22 g/ml fractions. The d < 1.22 g/ml fraction produced by PLTP was larger, had lower apolipoprotein A-I and higher lipid and apolipoprotein A-II content than native HDL2. The d > 1.22 g/ml fraction represented 30% of the initial HDL2 protein and consisted of small, apolipoprotein A-I and phospholipid-rich particles, with a high sphingomyelin:phosphatidylcholine ratio. Incubation with H-TGL led to a d < 1.22 g/ml fraction which was comparable to native HDL2 regarding size and chemical composition. The d > 1.22 g/ml particles represented only 5% of the initial HDL2 protein and had slightly higher diameter and sphingomyelin:phosphatidylcholine ratio than those produced by PLTP. Enrichment of HDL2 with triglyceride prior to incubation increased the amount of protein released into the d > 1.22 g/ml fraction (20%) but had no effect on size and chemical composition of the particles. We conclude that PLTP and H-TGL promote the formation of small, pre-beta-like HDL particles from HDL2.
将高密度脂蛋白2（HDL2）与磷脂转运蛋白（PLTP）或肝脂酶（H-TGL）一起孵育，然后将孵育产物分离成密度小于1.22 g/ml和密度大于1.22 g/ml的组分。由PLTP产生的密度小于1.22 g/ml的组分比天然HDL2更大，载脂蛋白A-I含量更低，脂质和载脂蛋白A-II含量更高。密度大于1.22 g/ml的组分占初始HDL2蛋白的30%，由小的、富含载脂蛋白A-I和磷脂的颗粒组成，鞘磷脂与磷脂酰胆碱的比例较高。与H-TGL孵育产生的密度小于1.22 g/ml的组分在大小和化学组成方面与天然HDL2相当。密度大于1.22 g/ml的颗粒仅占初始HDL2蛋白的5%，其直径和鞘磷脂与磷脂酰胆碱的比例略高于由PLTP产生的颗粒。孵育前用甘油三酯富集HDL2会增加释放到密度大于1.22 g/ml组分中的蛋白量（20%），但对颗粒的大小和化学组成没有影响。我们得出结论，PLTP和H-TGL促进了由HDL2形成小的、前β样HDL颗粒。
