Suthipintawong C, Leong A S, Chan K W, Vinyuvat S
Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, Hong Kong.
Diagn Cytopathol. 1997 Aug;17(2):127-33. doi: 10.1002/(sici)1097-0339(199708)17:2<127::aid-dc8>3.0.co;2-d.
An effective but simple fixation protocol for the immunocytochemical staining of cytologic smears for estrogen and progesterone receptors, the Ki-67 antigen (using MIB1 antibody), and c-erbB-2 protein is described. One hundred twenty-seven smears from a variety of malignant and benign breast lesions showed good preservation of antigenicity when subjected to the following fixation protocol: Freshly made smears were air-dried for 20 min to 14 h at 22 degrees C before immersing in 10% buffered formalin for 2-14 h. Immunostaining followed microwave-stimulated epitope retrieval. There was strong concordance of staining with corresponding tissue sections in 15 cases of malignant tumors (ER: r = 0.7381; PR: r = 0.6684; MIB1: r = 0.7234). Immunostaining staining, when delayed for 5-10 days in about half the smears, showed no noticeable difference in reactivity, attesting to effective storage of the formalin-fixed smears at room temperature.
本文描述了一种用于雌激素和孕激素受体、Ki-67抗原（使用MIB1抗体）以及c-erbB-2蛋白的细胞涂片免疫细胞化学染色的有效且简单的固定方案。来自各种恶性和良性乳腺病变的127份涂片在经过以下固定方案处理后，抗原性得到了良好保存：新鲜制备的涂片在22℃下风干20分钟至14小时，然后浸入10%缓冲福尔马林中2至14小时。免疫染色采用微波刺激抗原修复。15例恶性肿瘤病例的涂片与相应组织切片的染色具有高度一致性（雌激素受体：r = 0.7381；孕激素受体：r = 0.6684；MIB1：r = 0.7234）。约一半的涂片免疫染色延迟5至10天，反应性无明显差异，证明福尔马林固定的涂片在室温下可有效保存。
