Hynes N M, Bain J R, Thoma A, Veltri K, Maguire J A
Department of Surgery, McMaster University, Hamilton, Ontario, Canada.
J Reconstr Microsurg. 1997 Jul;13(5):337-43. doi: 10.1055/s-2007-1006413.
The goal of this study was to determine whether sensory motor nerve crossover could alter post-denervation atrophy of skeletal muscle. Sixty adult Lewis rats were divided into three groups: 1) unilateral transection of the tibial nerve alone; 2) unilateral transection of the tibial nerve with immediate repair; and 3) unilateral tibial and sural nerve transections with repair of the proximal sural nerve (sensory) to the distal tibial nerve (motor). The unoperated hind legs acted as positive controls. At 1 and 2 months postoperatively, posterior compartment musculature was harvested, weighed, then fixed and stained for histologic analysis. One month postoperatively, mean muscle weight in Group 1 animals (transection alone) was 23.0 +/- 2.6 percent of the control side; for Group 2 animals (motor-motor repair) was 40.9 +/- 42 percent; and for the sensory-protected Group 3 animals (sensory-motor repair) was 26.7 +/- 2.8 percent of controls (n = 15 per group). Two months postoperatively, the mean weights were 14.5 +/- 0.9 percent, 58.8 +/- 7.3 percent, and 21.1 +/- 3.1 percent of controls for Groups 1, 2, and 3, respectively (n = 5 per group). Differences between groups were statistically significant. Histologic analysis of Group 1 specimens revealed generalized atrophy of all muscle fibers. In Group 2, specimens showed evidence of reinnervation and less atrophy. Group 3 specimens demonstrated an atrophic pattern with islands of non-atrophic fibers scattered throughout. Sensory protection was thus shown to have significant effect on post-denervation atrophy in rat skeletal muscle.
本研究的目的是确定感觉运动神经交叉是否会改变骨骼肌去神经支配后的萎缩。60只成年Lewis大鼠被分为三组：1）单纯单侧切断胫神经；2）单侧切断胫神经并立即修复；3）单侧切断胫神经和腓肠神经，并将近端腓肠神经（感觉神经）修复至远端胫神经（运动神经）。未手术的后腿作为阳性对照。术后1个月和2个月，取后肢肌群，称重，然后固定并染色进行组织学分析。术后1个月，第1组动物（单纯切断组）的平均肌肉重量为对照侧的23.0±2.6%；第2组动物（运动-运动修复组）为40.9±4.2%；第3组感觉保护组动物（感觉-运动修复组）为对照侧的26.7±2.8%（每组n = 15）。术后2个月，第1、2、3组的平均重量分别为对照侧的14.5±0.9%、58.8±7.3%和21.1±3.1%（每组n = 5）。组间差异具有统计学意义。第1组标本的组织学分析显示所有肌纤维均出现广泛性萎缩。第2组标本显示有再支配的迹象且萎缩较轻。第3组标本表现为萎缩模式，有散在分布的非萎缩性纤维岛。因此，感觉保护对大鼠骨骼肌去神经支配后的萎缩有显著影响。
