Scherzer C R, Landwehrmeyer G B, Kerner J A, Standaert D G, Hollingsworth Z R, Daggett L P, Veliçelebi G, Penney J B, Young A B
Department of Neurology, Massachusetts General Hospital, Boston 02114, USA.
Neurobiol Dis. 1997;4(1):35-46. doi: 10.1006/nbdi.1997.0136.
We have used a quantitative in situ hybridization method with human ribonucleotide probes to examine the regional and cellular distribution of N-methyl-D-aspartate receptor (NMDAR) subunit mRNAs in the human cerebellum. Purkinje cells showed very dense labeling for NMDAR1 mRNA, dense labeling for NMDAR2A mRNA, and moderate labeling for NMDAR2D mRNA, whereas labeling for NMDAR2C mRNA was low. Granule cells showed high hybridization signals for the NMDAR1 and NMDAR2C mRNAs and moderate signals for the NMDAR2A and NMDAR2D mRNAs. In addition intense labeling with the NMDAR2B probe was observed in medium-sized neurons with chromophilic cell bodies in the upper part of the granule cell layer, most likely representing Golgi cells. Neurons in the molecular layer, i.e., basket cells and stellate cells, showed moderate hybridization signals for NMDAR1 and NMDAR2D and low signal for NMDAR2C. Each type of cerebellar neuron analyzed displayed a distinct NMDAR2 subunit profile, suggesting that they are likely to have NMDA receptors with distinct properties.
我们使用了一种定量原位杂交方法，利用人核糖核苷酸探针来检测人小脑中N-甲基-D-天冬氨酸受体（NMDAR）亚基mRNA的区域和细胞分布。浦肯野细胞对NMDAR1 mRNA呈非常密集的标记，对NMDAR2A mRNA呈密集标记，对NMDAR2D mRNA呈中度标记，而对NMDAR2C mRNA的标记较低。颗粒细胞对NMDAR1和NMDAR2C mRNAs呈高杂交信号，对NMDAR2A和NMDAR2D mRNAs呈中度信号。此外，在颗粒细胞层上部具有嗜色性细胞体的中型神经元中观察到用NMDAR2B探针进行的强烈标记，最有可能代表高尔基细胞。分子层中的神经元，即篮状细胞和星状细胞，对NMDAR1和NMDAR2D呈中度杂交信号，对NMDAR2C呈低信号。分析的每种类型的小脑神经元都显示出独特的NMDAR2亚基谱，表明它们可能具有具有不同特性的NMDA受体。
