Obál F, Bodosi B, Szilágyi A, Kacsóh B, Krueger J M
Department of Physiology, A. Szent-Györgyi Medical University, Szeged, Hungary.
Neuroendocrinology. 1997 Jul;66(1):9-16. doi: 10.1159/000127213.
To determine whether an acute withdrawal of growth hormone (GH) alters sleep, the effects of antiserum to GH (GH-AS) on sleep were studied in the rat. Sleep-wake activity and cortical brain temperature (Tc) were recorded for 2 days after systemic injection of physiological saline. Then, one group of rats (n = 6) received GH-AS whereas another group of rats was injected with normal rabbit serum (n = 6). The injections were given 1 h before light onset, and the rats' behaviors were recorded for 23 h during the subsequent 12-hour light and 12-hour dark period. Sleep and Tc were not altered after normal rabbit serum. The durations of both rapid eye movement sleep (REMS) and non-REMS (NREMS), and the EEG slow-wave activity during NREMS were significantly suppressed during the light period following the injection of GH-AS. Tc tended to decrease for 3 h and a small rise was observed thereafter during the light period, but these changes were not statistically significant. The assay of GH in plasma samples obtained at 30-min intervals for 5 h after injection of normal rabbit serum or GH-AS verified the decreases in plasma GH concentrations in response to GH-AS. It is suggested that GH may promote sleep possibly via some metabolic actions.
为了确定生长激素（GH）的急性撤除是否会改变睡眠，研究了抗生长激素血清（GH-AS）对大鼠睡眠的影响。在全身注射生理盐水后记录2天的睡眠-觉醒活动和大脑皮质温度（Tc）。然后，一组大鼠（n = 6）接受GH-AS，而另一组大鼠注射正常兔血清（n = 6）。在光照开始前1小时进行注射，并在随后12小时光照和12小时黑暗期的23小时内记录大鼠的行为。注射正常兔血清后，睡眠和Tc未发生改变。注射GH-AS后的光照期，快速眼动睡眠（REMS）和非快速眼动睡眠（NREMS）的时长以及NREMS期间的脑电图慢波活动均受到显著抑制。在光照期，Tc有3小时呈下降趋势，之后有小幅上升，但这些变化无统计学意义。在注射正常兔血清或GH-AS后，每隔30分钟采集一次血浆样本，共采集5小时，对血浆中GH的检测证实了注射GH-AS后血浆GH浓度的降低。提示GH可能通过某些代谢作用促进睡眠。
