Dombrowski L J, Browning R S, Pratt E L
J Pharm Sci. 1977 Oct;66(10):1413-5. doi: 10.1002/jps.2600661016.
A direct spectrophotometric method for the quantitative determination of sulfathiazole and the total pyrimidyl sulfonamide content in a mixture containing sulfadiazine and sulfamerazine is reported. This three-component system was readily reduced to the problem of a simple two-component system analysis. Based on dual isoabsorptive wavelength spectroscopy, simultaneous equations were developed that required absorbance measurements at only two selected wavelengths both isoabsorptive. The location of the isoabsorptive wavelengths was dependent on the pH of the solvent medium, and 0.1 M HCl gave the best results. The validity of the derived equations was demonstrated in a recovery study involving synthetic mixtures containing varying quantities of the three sulfonamides. The recovery was linear over a wide concentration range, and the precision of the method was about 1%.
报道了一种直接分光光度法，用于定量测定含有磺胺嘧啶和磺胺甲基嘧啶的混合物中磺胺噻唑及总嘧啶基磺胺的含量。这个三元体系很容易简化为简单的二元体系分析问题。基于双等吸收波长光谱法，建立了仅需在两个选定的等吸收波长处测量吸光度的联立方程。等吸收波长的位置取决于溶剂介质的pH值，0.1 M盐酸给出了最佳结果。在一项涉及含有不同量三种磺胺的合成混合物的回收率研究中，证明了所推导方程的有效性。回收率在很宽的浓度范围内呈线性，该方法的精密度约为1%。
